Description

8-((4-Bromo-2,3-Dioxobutyl)Thio)Adenosine 5'-Triphosphate is a chemically modified ATP analog designed as a potent and selective affinity label for nucleotide-binding proteins. This compound matters as a critical biochemical tool for probing enzyme active sites, studying kinase mechanisms, and developing targeted covalent inhibitors. Researchers in biochemistry, enzymology, and medicinal chemistry need this high-purity reagent for advanced studies in signal transduction and drug discovery.

Application

• Enzyme Active Site Labeling: Acts as an affinity label to covalently modify and identify nucleotide-binding domains in kinases and ATPases.
• Mechanistic Biochemistry Studies: Used to investigate the catalytic mechanisms of enzymes that utilize ATP as a substrate or cofactor.
• Proteomics Research: Employed in chemical proteomics to profile and enrich ATP-binding proteins from complex biological samples.
• Covalent Inhibitor Development: Serves as a key precursor or model compound for designing irreversible inhibitors targeting metabolic and signaling pathways.
• Structural Biology: Aids in X-ray crystallography and structural studies by trapping enzyme-substrate complexes.
• Diagnostic Assay Development: Utilized in the development of specialized assays for enzyme activity and high-throughput screening.

Basic Information

Product Name	8-((4-Bromo-2,3-Dioxobutyl)Thio)Adenosine 5'-Triphosphate
CAS No.	115678-79-0
Molecular Formula	C14H19BrN5O13P3S
Molecular Weight	695.27 g/mol
Synonyms	8-[(4-Bromo-2,3-dioxobutyl)thio]ATP; 8-[(4-Bromo-2,3-dioxobutyl)thio]adenosine-5'-triphosphate; BDB-T-ATP; 8-(4-Bromo-2,3-dioxobutylthio)ATP; Adenosine 5'-(tetrahydrogen triphosphate), 8-[(4-bromo-2,3-dioxobutyl)thio]-; 8-BDBT-ATP; ATP Affinity Label; Covalent ATP Analog
EINECS	Contact for details

Quality Control

Our 8-((4-Bromo-2,3-Dioxobutyl)Thio)Adenosine 5'-Triphosphate is manufactured under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability data. We adhere to ISO 9001 quality management principles, and our analytical methods, including HPLC and NMR, are validated to confirm product integrity.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO, or buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.