Description

5-(3-Amino-1-Propynyl)-2',3'-Dideoxyuridine,5'-Triphosphate is a chemically modified nucleoside triphosphate analog, a critical building block for advanced biochemical research and development. Its primary value lies in its role as a chain terminator and substrate analog in enzymatic studies, particularly for reverse transcriptase and DNA polymerase assays. This compound is essential for researchers and manufacturers in the fields of antiviral drug discovery, molecular biology, and diagnostic kit development, where precise nucleotide analogs are required to study viral replication mechanisms and develop novel therapeutic agents.

Application

• Antiviral Drug Discovery: Used as a substrate analog to screen and evaluate potential inhibitors of viral polymerases, particularly for HIV and other retroviruses.
• Molecular Biology Research: Serves as a chain-terminating nucleotide in DNA sequencing techniques and polymerase activity assays.
• Biochemical Probe Development: Incorporated into oligonucleotides to create probes for studying enzyme kinetics and mechanisms of nucleic acid synthesis.
• Diagnostic Assay Manufacturing: A key raw material in the production of kits for detecting viral load and studying drug resistance mutations.
• Enzymology Studies: Utilized to investigate the substrate specificity and catalytic properties of DNA-dependent and RNA-dependent polymerases.

Basic Information

Product Name	5-(3-Amino-1-Propynyl)-2',3'-Dideoxyuridine,5'-Triphosphate
CAS No.	114748-59-3
Molecular Formula	C₁₃H₁₈N₄O₁₃P₃
Molecular Weight	564.22 g/mol
Synonyms	5-(3-Aminoprop-1-yn-1-yl)-2',3'-dideoxyuridine-5'-triphosphate; 5-(3-Amino-1-propynyl)-2',3'-dideoxy-UTP; 5-(3-Aminopropynyl)-ddUTP; 5-AP-ddUTP; 5-(3-Aminoprop-1-ynyl)-2',3'-dideoxyuridine triphosphate; Modified Nucleotide Triphosphate; Chain Terminator Nucleotide; Antiviral Nucleotide Analog
EINECS	Contact for details

Quality Control

Our production of 5-(3-Amino-1-Propynyl)-2',3'-Dideoxyuridine,5'-Triphosphate adheres to stringent quality protocols suitable for research and diagnostic applications. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided to ensure traceability and compliance with your research specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to keep the material desiccated and under an inert atmosphere to maintain stability and potency.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.