Description

Citric Acid Trisodium Salt Hydrate, Dnase, Rnase And Protease Free, For Molecular Biology, 99.8% is a high-purity, ultra-clean buffer salt specifically engineered to eliminate enzymatic contamination that can compromise sensitive molecular biology experiments. This product is critical for researchers and manufacturers who require absolute reliability in applications where nucleic acid integrity is paramount. It is an essential reagent for life science research, diagnostic assay development, and biopharmaceutical production processes.

Application

• Molecular Biology Buffers: A key component in high-fidelity buffer systems for PCR, RT-PCR, DNA/RNA extraction, purification, and sequencing where nuclease and protease activity must be excluded.
• Cell Culture & Bioprocessing: Used in the formulation of cell culture media and fermentation broths, providing pH control without introducing enzymatic contaminants that affect cell growth or product yield.
• Diagnostic Reagents: Integral to the manufacturing of diagnostic kits (e.g., ELISA, lateral flow assays) requiring stable, contaminant-free buffer conditions to ensure assay specificity and sensitivity.
• Protein Biochemistry: Serves as a buffering agent in protein crystallization, electrophoresis, chromatography, and storage where proteolytic degradation must be prevented.
• Next-Generation Sequencing (NGS): Employed in library preparation and other NGS workflow steps that demand ultra-pure reagents to prevent sample degradation and ensure accurate data.
• Pharmaceutical Formulations: Used as an excipient or buffering agent in parenteral and ophthalmic solutions where purity and absence of biological contaminants are critical for safety.

Basic Information

Product Name	Citric Acid Trisodium Salt Hydrate, Dnase, Rnase And Protease Free, For Molecular Biology, 99.8%
CAS No.	114456-61-0
Molecular Formula	C6H5Na3O7 · xH2O
Molecular Weight	294.10 g/mol (anhydrous basis)
Synonyms	Trisodium Citrate Dihydrate (Molecular Biology Grade); Sodium Citrate Tribasic Dihydrate (DNase, RNase, Protease free); Citrosodine; NSC 2823; 1,2,3-Propanetricarboxylic acid, 2-hydroxy-, trisodium salt, hydrate; E331; Trisodium 2-hydroxypropane-1,2,3-tricarboxylate hydrate
EINECS	Contact for details

Quality Control

This molecular biology grade product is manufactured under controlled conditions to meet the stringent purity requirements of life science applications. Every batch is tested to be free of detectable DNase, RNase, and protease activities. Certificates of Analysis (COA) are provided, detailing specifications for assay, identification, pH, clarity of solution, heavy metals, and bioburden. Our quality system ensures traceability and consistency for critical research and GMP-grade manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive). Keep the container tightly sealed in a desiccated environment to prevent absorption of atmospheric moisture, which can affect weight and performance.

Specification

Item	Specification
Appearance	White crystalline powder
Identification (IR)	Conforms
Assay (Titration)	99.8% min
pH (5% solution)	7.5 - 9.0
Clarity of Solution	Clear, colorless
DNase Activity	Not detected
RNase Activity	Not detected
Protease Activity	Not detected
Heavy Metals (as Pb)	< 5 ppm
Endotoxin (LAL)	< 0.25 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.