Description

3'-O-((N,N-Diisopropylamino)(2-Cyanoethoxy)Phosphinyl)-5'-O-(9-Phenylxanthen-9-Yl)-2'-Deoxynebularine is a highly specialized phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. Its value lies in enabling the precise and efficient incorporation of nebularine analogs, which are nucleoside derivatives with unique base-pairing properties, into synthetic DNA strands. This compound is essential for researchers and manufacturers in pharmaceutical development, molecular diagnostics, and academic research who are developing novel antisense oligonucleotides, aptamers, or probes for therapeutic and diagnostic applications.

Application

• Oligonucleotide Synthesis: Key phosphoramidite monomer for the automated solid-phase synthesis of DNA strands containing 2'-deoxynebularine modifications.
• Antisense Drug Development: Used in research and pre-clinical production of antisense oligonucleotides (ASOs) targeting specific mRNA sequences.
• Aptamer Production: Enables the creation of synthetic DNA or RNA aptamers with enhanced binding affinity and specificity for target proteins or molecules.
• Molecular Probe Design: Critical for manufacturing labeled probes and primers used in PCR, sequencing, and hybridization assays that require modified bases.
• Structural Biology Studies: Used to synthesize oligonucleotides for studying nucleic acid structure, function, and protein-nucleic acid interactions.
• Therapeutic Nucleic Acid Research: A vital reagent in the development of novel therapeutic modalities including siRNA, miRNA, and gene editing components.

Basic Information

Product Name	3'-O-((N,N-Diisopropylamino)(2-Cyanoethoxy)Phosphinyl)-5'-O-(9-Phenylxanthen-9-Yl)-2'-Deoxynebularine
CAS No.	114155-97-4
Molecular Formula	C41H47N6O6P
Molecular Weight	750.83 g/mol
Synonyms	2'-Deoxynebularine 3'-CE Phosphoramidite; 9-Phenylxanthen-9-yl (PX) protected 2'-deoxynebularine 3'-phosphoramidite; Nebularine (2'-deoxy) 3'-phosphoramidite; 5'-O-(9-Phenylxanthen-9-yl)-2'-deoxynebularine 3'-O-((2-Cyanoethyl) (N,N-diisopropyl)phosphoramidite); DNe 3'-Phosphoramidite; 2'-Deoxynebularine, 5'-O-(9-phenyl-9H-xanthen-9-yl)-, 3'-[2-cyanoethyl N,N-diisopropylphosphoramidite]
EINECS	Contact for details

Quality Control

Every batch of our phosphoramidite monomers is produced and tested under strict quality management systems to ensure high purity and batch-to-batch consistency, critical for successful oligonucleotide synthesis. Our standard quality assurance includes rigorous analysis by HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment. We support development under cGMP guidelines for advanced therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dedicated freezer. This product is hygroscopic (moisture-sensitive) and easily oxidized; allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. Use immediately after opening or re-seal under inert gas.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.