Description

Da-Me Phosphonamidite CAS NO 114079-05-9 is a specialized organophosphorus compound, specifically a phosphoramidite derivative, widely used as a key building block in oligonucleotide synthesis. Its primary value lies in its role as a crucial monomer for the automated, solid-phase synthesis of DNA and RNA sequences, enabling precise control over genetic material assembly. This high-purity reagent is essential for research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in developing antisense therapies, PCR primers, and genetic probes.

Application

• Oligonucleotide Synthesis: Serves as a critical phosphoramidite monomer in automated solid-phase synthesizers for the production of DNA and RNA strands.
• Antisense & siRNA Therapeutics: Used in the manufacture of therapeutic oligonucleotides for gene silencing and targeted drug development.
• Diagnostic Probe Manufacturing: Essential for creating labeled probes used in PCR, qPCR, FISH, and microarray technologies for disease detection.
• Research & Development: A fundamental reagent in academic and industrial labs for studying gene function, synthetic biology, and nucleic acid chemistry.
• Next-Generation Sequencing (NGS): Employed in the synthesis of primers and adapters required for library preparation in genomic sequencing platforms.
• CRISPR-Cas9 Guide RNA Synthesis: Used in the production of synthetic guide RNAs (sgRNAs) for genome editing applications.

Basic Information

Product Name	Da-Me Phosphonamidite
CAS No.	114079-05-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Phosphoramidite monomer; DNA Phosphoramidite; RNA Phosphoramidite; Nucleoside Phosphoramidite; Da-Me Amidite; 5'-O-Dimethoxytrityl-N-acyl-2'-deoxynucleoside-3'-O-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite (generic IUPAC-type name); CE Phosphoramidite; Gene synthesis reagent
EINECS	Contact for details

Quality Control

Our Da-Me Phosphonamidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, critical for successful oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC for purity and 31P NMR for structural confirmation, to meet the stringent requirements of pharmaceutical and diagnostic applications. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a recommended temperature of -20°C or below under an inert atmosphere (argon or nitrogen) due to its moisture-sensitive and light-sensitive nature. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms
Identification (31P NMR)	Conforms
Purity (HPLC)	≥ 98.5%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.