Description

2'-3'-o-(4-Benzoylbenzoyl)Guanosine*5'-Triphosphate CAS NO 112898-14-3 is a chemically modified nucleotide analog, specifically a caged GTP derivative, designed for precise photochemical control in biochemical research. This compound is critical for researchers who require temporally and spatially controlled release of the biologically active GTP molecule upon exposure to UV light. It is an essential tool for studying dynamic cellular processes such as G-protein signaling, microtubule polymerization, and small GTPase function in real-time.

Application

• Photolysis Studies: Used as a "caged" compound to release GTP upon UV flash photolysis for kinetic analysis of GTP-binding proteins.
• G-Protein & GTPase Research: Enables precise activation of heterotrimeric G-proteins, Ras, Rho, and Rab family GTPases in live cells or reconstituted systems.
• Cytoskeleton Dynamics: Investigates the real-time assembly and disassembly of microtubules by controlling the availability of GTP for tubulin polymerization.
• Neuroscience & Signal Transduction: Studies rapid signaling events in neurons and other cell types where traditional agonist application is too slow.
• Enzyme Mechanism Studies: Provides insights into the catalytic mechanisms of enzymes that utilize GTP, such as kinases and synthases.
• High-Resolution Cellular Imaging: Allows for localized activation of GTP-dependent pathways to map signaling networks within specific cellular compartments.

Basic Information

Product Name	2'-3'-o-(4-Benzoylbenzoyl)Guanosine*5'-Triphosphate
CAS No.	112898-14-3
Molecular Formula	C28H28N5O18P3
Molecular Weight	839.47 g/mol
Synonyms	Bz2-caged GTP; 2',3'-O-(4-Benzoylbenzoyl)guanosine 5'-triphosphate; (2',3'-O-(4-Benzoylbenzoyl))GTP; Caged GTP, (2',3'-(4-Benzoylbenzoyl)); Guanosine 5'-triphosphate, 2',3'-O-(4-benzoylbenzoyl)-; P3-(2',3'-O-(4-Benzoylbenzoyl)guanosin-5'-yl) P1,P2-diphosphate triester; Photolabile GTP analog
EINECS	Contact for details

Quality Control

Our 2'-3'-o-(4-Benzoylbenzoyl)Guanosine*5'-Triphosphate is produced under stringent conditions to ensure the highest standard of purity and biological activity. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment, NMR for structural confirmation, and functional validation in photolysis assays. Certificates of Analysis (COA) detailing purity, identity, and performance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Photolytic Efficiency	≥ 70% release of GTP upon UV irradiation
Biological Activity	Functional in standard caged nucleotide assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.