Description

n-Blocked-5'-o-Dmt-2'-o-Me Ced Cytosine Phosphoramidite CAS NO 110764-78-8 is a high-purity, protected nucleoside phosphoramidite monomer, a critical building block for the solid-phase synthesis of oligonucleotides. Its value lies in enabling the precise and efficient incorporation of modified cytosine analogs into synthetic DNA and RNA strands, which is essential for advanced research and therapeutic development. This product is primarily needed by biotechnology and pharmaceutical companies engaged in antisense therapy, siRNA development, diagnostic probe synthesis, and other cutting-edge nucleic acid applications.

Application

• Oligonucleotide Synthesis: As a key monomer for the automated solid-phase synthesis of DNA and RNA strands containing modified cytidine.
• Antisense Oligonucleotide (ASO) Production: For creating therapeutic oligonucleotides designed to modulate gene expression by binding to specific RNA targets.
• Small Interfering RNA (siRNA) Manufacturing: Used in the synthesis of siRNA duplexes for RNA interference (RNAi) research and therapeutic development.
• Diagnostic Probe Development: Essential for producing labeled or modified probes used in PCR, FISH, microarray, and other molecular diagnostic assays.
• Aptamer and SELEX Technology: For constructing libraries of synthetic oligonucleotides with modified bases to select for high-affinity binding molecules.
• Basic Biochemical Research: Enables the study of nucleic acid structure, function, and protein interactions by incorporating site-specific modifications.

Basic Information

Item	Detail
Product Name	n-Blocked-5'-o-Dmt-2'-o-Me Ced Cytosine Phosphoramidite
CAS No.	110764-78-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-methylcytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-Methyl-5'-O-DMT-cytidine-3'-CE-phosphoramidite; 2'-O-Me-Ced Phosphoramidite; DMT-2'-O-methylcytidine CE phosphoramidite; 2'-O-Methyl-C Phosphoramidite; 5'-Dimethoxytrityl-2'-O-methylcytidine-3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); 2'-O-Methyl-Ced Phosphoramidite
EINECS	Contact for details

Quality Control

Our n-Blocked-5'-o-Dmt-2'-o-Me Ced Cytosine Phosphoramidite is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch undergoes comprehensive analytical testing, including HPLC for purity and assay, 31P NMR and 1H NMR for structural confirmation, and moisture content analysis (KF). Certificates of Analysis (COA) with full specification data are provided to guarantee compliance with research and cGMP-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions, moisture, or light will lead to decomposition and reduced coupling efficiency. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Assay	≥ 97.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.