Description

N(4)-(6-Aminohexyl)Deoxycytidine 5'-Triphosphate is a chemically modified nucleotide triphosphate analog featuring a primary amine linker at the N4 position of the cytosine base. This modification is critical for its primary use as a key reagent in molecular biology and diagnostic applications, enabling covalent attachment to various probes and surfaces. It is essential for researchers and manufacturers in the fields of next-generation sequencing (NGS), microarray fabrication, and advanced molecular diagnostics who require high-purity, functionalized nucleotides for labeling and conjugation.

Application

• DNA Labeling and Probe Synthesis: Used for enzymatic incorporation of amine-modified nucleotides, creating labeled DNA for hybridization assays.
• Next-Generation Sequencing (NGS): Serves as a substrate for DNA polymerases in sequencing-by-synthesis platforms requiring modified nucleotides.
• Microarray and Biochip Fabrication: The primary amine group allows for covalent immobilization of oligonucleotides onto activated glass or polymer surfaces.
• Diagnostic Assay Development: A key component in the production of conjugated probes for fluorescence in situ hybridization (FISH), qPCR, and other molecular tests.
• Bioconjugation: The reactive amine handle facilitates cross-linking to enzymes (e.g., HRP), fluorescent dyes, biotin, or other reporter molecules.
• Structural Biology Studies: Used to introduce specific modifications into DNA for studying protein-DNA interactions or nucleic acid structure.

Basic Information

Product Name	N(4)-(6-Aminohexyl)Deoxycytidine 5'-Triphosphate
CAS No.	110537-14-9
Molecular Formula	C16H28N5O13P3
Molecular Weight	615.34 g/mol
Synonyms	6-((4-Amino-2-oxo-1(2H)-pyrimidinyl)methylamino)hexyl trihydrogen triphosphate; dCTP-6-NH2; Aminoallyl-dCTP; AA-dCTP; 5'-Triphosphoric acid, P'→5'-ester with 1-(4-amino-2-oxo-1(2H)-pyrimidinyl)-1-deoxy-6-(hexylamino)-D-ribofuranose; N4-(6-Aminohexyl)-2'-deoxycytidine-5'-triphosphate; Amine-modified dCTP
EINECS	Contact for details

Quality Control

Our N(4)-(6-Aminohexyl)Deoxycytidine 5'-Triphosphate is manufactured under strict quality systems. Each batch is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and absence of contaminating nucleotides is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5%
Enzymatic Incorporation Test	Suitable for use

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.