Description

5-(3-Aminoallyl)-2'-Deoxy-Uridine 5'-Triphosphate Sodium Salt is a chemically modified nucleotide triphosphate essential for advanced molecular biology techniques. Its core value lies in the aminoallyl group, which serves as a reactive handle for efficient labeling with fluorescent dyes or other probes. This compound is critically needed by researchers and diagnostic developers in life sciences, particularly for applications in next-generation sequencing (NGS), microarray fabrication, and fluorescence in situ hybridization (FISH).

Application

• Fluorescent Labeling for Microarrays: The primary amine group facilitates covalent coupling to NHS-ester dyes (e.g., Cy3, Cy5) for the synthesis of labeled nucleotides used in DNA microarray experiments.
• Next-Generation Sequencing (NGS): Acts as a key reagent for incorporating labeled nucleotides during sequencing-by-synthesis, enabling real-time detection and high-throughput genomic analysis.
• Fluorescence In Situ Hybridization (FISH): Used to prepare brightly labeled nucleic acid probes with enhanced stability for chromosomal analysis and pathogen detection.
• Single-Molecule Detection: Essential for synthesizing nucleotides labeled with bright, photostable fluorophores for super-resolution microscopy and single-molecule tracking studies.
• Probe Synthesis for Diagnostics: Manufacture of highly sensitive and specific detection probes for clinical diagnostic kits, including PCR-based assays.
• Bioconjugation & Chemoproteomics: Serves as a versatile building block for creating bioconjugates or for cross-linking studies in proteomics research.

Basic Information

Product Name	5-(3-Aminoallyl)-2'-Deoxy-Uridine 5'-Triphosphate Sodium Salt
CAS No.	109921-28-0
Molecular Formula	C13H19N3O14P3Nan (n≈3)
Molecular Weight	~665.1 (Free acid, anhydrous basis)
Synonyms	AA-dUTP; 5-(3-Aminoallyl)-dUTP; Aminoallyl-dUTP; 5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, trisodium salt; 5-(3-Aminoprop-2-en-1-yl)-2'-deoxyuridine-5'-triphosphate; 5-AA-dUTP; 5-(3-Aminoallyl)-dUTP, Na salt; 5-(3-Aminoallyl)-2'-deoxyuridine triphosphate
EINECS	Contact for details

Quality Control

Our 5-(3-Aminoallyl)-dUTP is manufactured under stringent conditions to ensure the highest purity and batch-to-batch consistency, critical for sensitive labeling reactions. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and mass spectrometry for identity confirmation. A detailed Certificate of Analysis (COA) is provided, documenting purity, concentration, and absence of contaminating nucleases and phosphatases.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider desiccant and under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Nucleotide basis)
Assay (UV Spectroscopy)	≥ 90% (as sodium salt, anhydrous basis)
Solubility	Soluble in water (pH ~7.0)
Extinction Coefficient (ε260)	~8,700 M-1cm-1 (in H2O, pH 7.0)
pH (1% Solution)	6.5 - 7.5
Nuclease Activity	Not detected
Phosphatase Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.