Description

(2-Cyanoethoxy)-2-(2''-o-1,1''-Dimethoxytrityloxyethylsulfonyl)- Ethoxy-N,n-Diisopropylaminophosphine) is a high-purity phosphoramidite monomer, a critical building block in the automated synthesis of oligonucleotides. Its value lies in enabling the precise and efficient formation of internucleotide linkages, which is fundamental for creating DNA and RNA sequences with high fidelity. This compound is essential for researchers and manufacturers in the fields of molecular biology, diagnostics, and therapeutic development who require reliable, high-quality reagents for solid-phase oligonucleotide synthesis.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer in automated solid-phase synthesis for research-scale and industrial-scale production of DNA and RNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides (ASOs), small interfering RNA (siRNA), and other nucleic acid-based therapeutics.
• Diagnostic Probe Development: Synthesis of labeled probes for techniques like PCR, FISH, and microarray analysis.
• Primer and Adaptor Synthesis: Manufacturing of primers for sequencing, cloning, and amplification applications.
• Antisense Research: Enabling the study of gene function and regulation through custom oligonucleotide synthesis.
• Synthesis of Modified Oligonucleotides: Used in the preparation of oligonucleotides containing specific backbone or sugar modifications for enhanced stability and specificity.

Basic Information

Item	Detail
Product Name	(2-Cyanoethoxy)-2-(2''-o-1,1''-Dimethoxytrityloxyethylsulfonyl)- Ethoxy-N,n-Diisopropylaminophosphine)
CAS No.	108783-02-4
Molecular Formula	C41H50N3O8PS
Molecular Weight	775.89 g/mol
Synonyms	5'-O-Dimethoxytrityl-N2-isobutyryl-2'-O-(2-methoxyethyl)-guanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-MOE RNA Phosphoramidite (Guanosine, DMTr, iBu); 2'-O-Methoxyethyl Guanosine Phosphoramidite; 5'-O-(4,4'-Dimethoxytrityl)-2'-O-(2-methoxyethyl)-N2-isobutyrylguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; RNA (2'-MOE) G Phosphoramidite; 2'-MOE-G Phosphoramidite; Anti-sense oligonucleotide synthesis monomer
EINECS	Contact for details

Quality Control

Our (2-Cyanoethoxy)-2-(2''-o-1,1''-Dimethoxytrityloxyethylsulfonyl)- Ethoxy-N,n-Diisopropylaminophosphine) is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, critical for sensitive oligonucleotide synthesis. Each lot undergoes rigorous analytical testing, including HPLC, NMR, and 31P NMR, to confirm identity, purity, and reactivity. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will lead to decomposition and reduced coupling efficiency.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.