Description

Restriction Endonuclease Vsp I is a high-purity, site-specific endonuclease enzyme used for precise DNA cleavage in molecular biology research and biotechnology applications. Its primary value lies in its ability to recognize and cut the specific DNA sequence ATTAAT, enabling critical workflows in genetic engineering, cloning, and genomic analysis. This enzyme is an essential reagent for molecular biologists, diagnostic kit manufacturers, and R&D laboratories in the pharmaceutical and life sciences sectors requiring reliable and consistent restriction digestion activity.

Application

• Molecular Cloning: Essential for the precise insertion of DNA fragments into vectors during recombinant DNA technology.
• Genomic DNA Analysis: Used in restriction fragment length polymorphism (RFLP) analysis and Southern blotting for genetic mapping and mutation detection.
• Diagnostic Assay Development: A key component in the manufacturing of diagnostic kits for genetic testing and pathogen identification.
• Plasmid DNA Mapping: Facilitates the verification and analysis of plasmid constructs by generating specific digestion patterns.
• Gene Synthesis and Assembly: Employed in modern synthetic biology techniques for the assembly of genetic circuits and pathways.
• Quality Control in Bioproduction: Used to check the integrity and identity of DNA-based products, including vaccines and therapeutics.

Basic Information

Product Name	Restriction Endonuclease Vsp I
CAS No.	108398-30-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	VspI; Endodeoxyribonuclease VspI; Type II restriction enzyme VspI; Restriction enzyme VspI; VspI restriction endonuclease; VspI endonuclease; ATTAAT-specific endonuclease
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Vsp I is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and optimal performance. Each lot is rigorously assessed for specific activity, purity (free from non-specific nucleases and phosphatases), and stability. A comprehensive Certificate of Analysis (COA) detailing activity units, purity assays, and storage buffer composition is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer; under recommended conditions, it maintains specified activity for extended periods.

Specification

Item	Specification
Appearance	Clear, colorless solution
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95% single band
Non-Specific Nuclease	Not detected
Phosphatase Activity	Not detected
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C
Optimal Incubation Temperature	37°C
Heat Inactivation	65°C for 20 minutes

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.