Description

2-Bromo-2'-Deoxyadenosine Triphosphate is a modified nucleotide triphosphate analog, a crucial building block for advanced molecular biology and biochemistry research. This compound is engineered to introduce specific functionalities or act as a chain terminator in enzymatic synthesis, enabling precise control over nucleic acid structure and function. It is primarily utilized by researchers and manufacturers in the fields of next-generation sequencing (NGS), polymerase chain reaction (PCR) probe development, and structural biology studies.

Application

• Next-Generation Sequencing (NGS): Acts as a substrate or terminator in sequencing-by-synthesis platforms for high-throughput genetic analysis.
• PCR and qPCR Probe Synthesis: Used in the enzymatic synthesis of labeled probes for real-time detection and quantification.
• Site-Directed Mutagenesis: Facilitates the introduction of specific mutations or modifications into DNA strands.
• Structural Studies (X-ray Crystallography/NMR): Incorporated into oligonucleotides to aid in determining three-dimensional macromolecular structures.
• Enzyme Mechanism Studies: Serves as a substrate analog to investigate the kinetics and specificity of DNA polymerases and other nucleic acid-processing enzymes.
• Aptamer and SELEX Technology: Used in the synthesis of modified nucleic acid libraries for the selection of high-affinity binding molecules.

Basic Information

Product Name	2-Bromo-2'-Deoxyadenosine Triphosphate
CAS No.	106867-29-2
Molecular Formula	C10H14BrN5O12P3
Molecular Weight	610.06 g/mol
Synonyms	2-Bromo-2'-deoxyATP; Br-dATP; 2-Br-dATP; 2-Bromo-2'-deoxyadenosine 5'-triphosphate; 2-Bromo-2'-deoxyadenosine-5'-triphosphate; 2-Bromo-deoxyadenosine triphosphate; 2-Br-2'-deoxyadenosine triphosphate; 2-Bromo-2'-deoxyadenosine-5'-(tetrahydrogen triphosphate)
EINECS	Contact for details

Quality Control

Our 2-Bromo-2'-Deoxyadenosine Triphosphate is produced under stringent quality management systems to ensure batch-to-batch consistency and high purity, essential for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation and storage under an inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 5.0 %
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.