Description

Biotin-14-Dctp is a biotin-labeled deoxycytidine triphosphate (dCTP) analog, a critical reagent for nucleic acid labeling and detection. This compound matters for researchers and diagnostic developers because it enables the specific, high-sensitivity tagging of DNA probes for applications like fluorescence in situ hybridization (FISH) and microarray analysis. Professionals in molecular biology research, in vitro diagnostics (IVD) manufacturing, and genomic service providers need it to create stable, non-radioactive detection systems.

Application

• Non-Radioactive Nucleic Acid Labeling: Primary use for incorporating biotin tags into DNA probes via enzymatic reactions like nick translation or random priming.
• Fluorescence In Situ Hybridization (FISH): Preparation of biotinylated probes for chromosome analysis and gene mapping, followed by detection with fluorescently labeled streptavidin.
• Microarray and Blotting Techniques: Labeling target DNA or RNA for hybridization on microarray chips or membranes (Southern, Northern blots).
• Diagnostic Assay Development: A key raw material for manufacturing IVD kits that utilize biotin-streptavidin chemistry for signal amplification.
• Next-Generation Sequencing (NGS) Library Prep: Potential use in certain protocols requiring modified nucleotides for capture or enrichment steps.
• Pull-Down Assays: Synthesis of biotinylated DNA baits for capturing protein or nucleic acid interactors using streptavidin-coated beads.

Basic Information

Item	Detail
Product Name	Biotin-14-Dctp
CAS No.	106519-39-5
Molecular Formula	C₂₅H₃₉N₆O₁₇P₃S
Molecular Weight	812.60 g/mol
Synonyms	Biotin-14-dCTP; Biotin-14-2'-deoxycytidine-5'-triphosphate; 2'-Deoxycytidine-5'-triphosphate, N⁴-[(6aS,9aS)-2-oxo-1,3,6a,8,9,9a-hexahydro-2H-furo[3',4':6,7]naphtho[2,3-d][1,3]dioxol-8-yl]-; Biotinylated dCTP; 14-Biotinyl-2'-deoxycytidine 5'-triphosphate; N⁴-(6-[Biotinylamido]hexyl)-2'-deoxycytidine 5'-triphosphate; Biotin-14-2'-dCTP; Biotin-XX-dCTP (related analog)
EINECS	Contact for details

Quality Control

Our Biotin-14-Dctp is manufactured under strict quality systems to ensure batch-to-batch consistency for critical research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and assay, verification of labeling efficiency, and tests for nuclease contamination. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting compliance with our stringent in-house specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (Enzymatic Incorporation)	≥ 90% incorporation efficiency
Water Content (KF)	≤ 5.0%
Solubility	Soluble in aqueous buffer (pH ~7.0)
Nuclease Contamination	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.