Description

5'-O-(4,4'-Dimethoxytrityl)-N6-Methyl-2'-Deoxyadenosine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the automated solid-phase synthesis of oligonucleotides. This compound is critical for researchers and manufacturers requiring precise incorporation of a modified adenine base, specifically N6-methyl-2'-deoxyadenosine, into DNA strands. It is primarily used by biotechnology, pharmaceutical, and diagnostic companies engaged in the development of therapeutic nucleic acids, primers, probes, and other advanced molecular biology tools.

Application

• Oligonucleotide Synthesis: As a key building block (phosphoramidite) in automated DNA synthesizers for the site-specific introduction of N6-methyladenosine (m6A) modifications.
• Therapeutic Nucleic Acid Development: For the production of modified antisense oligonucleotides (ASOs), siRNA, and aptamers where m6A modification can influence stability, specificity, and biological activity.
• Probe and Primer Manufacturing: Creating specialized DNA probes and primers for genomics research, PCR assays, and next-generation sequencing that require base-modified sequences.
• Epigenetics and RNA Mimicry Research: Synthesizing DNA strands that mimic RNA sequences containing m6A for studying methylation effects and protein-nucleic acid interactions.
• Diagnostic Kit Production: Used in the manufacture of high-fidelity components for molecular diagnostic tests and biosensors.
• Scale-Up GMP Manufacturing: Suitable for use in current Good Manufacturing Practice (cGMP) environments for the production of clinical-grade oligonucleotide APIs.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-N6-Methyl-2'-Deoxyadenosine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite
CAS No.	105931-58-6
Molecular Formula	C41H50N7O7P
Molecular Weight	783.87 g/mol
Synonyms	N6-Methyl-2'-deoxyadenosine 5'-O-(4,4'-dimethoxytrityl)-3'-[(2-cyanoethyl)-N,N-diisopropyl]phosphoramidite; N6-Methyl-dA Phosphoramidite; 5'-DMT-N6-Methyl-2'-deoxyadenosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; m6dA Phosphoramidite; N6-Methyladenine 2'-Deoxyriboside Phosphoramidite; DMTr-N6-Me-dA-CE Phosphoramidite; 2'-Deoxy-N6-methyladenosine phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of our phosphoramidite monomers is produced and tested under stringent quality management systems. Specifications typically meet or exceed 98.5% purity (HPLC), ensuring optimal coupling efficiency in oligonucleotide synthesis. Certificates of Analysis (COA) are provided with each shipment, detailing critical parameters such as assay, moisture content (KF), and identity confirmation (NMR, MS). Our quality commitment supports applications from research to GMP-grade manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Assay (HPLC)	≥ 98.5%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.