Description

3'-(2,2,5,5-Tetramethyl-3-Pyrroline-1-Oxyl-3-Carbonyl)Amino-3'-Deoxyguanosine 5'-Diphosphate is a specialized nucleotide analog incorporating a stable nitroxide radical, making it a critical spin-labeling reagent. This compound is essential for advanced research in structural biology and biophysics, particularly for probing nucleic acid dynamics and interactions using techniques like Electron Paramagnetic Resonance (EPR) spectroscopy. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic developers focused on nucleic acid structure-function studies and novel probe development.

Application

• Spin-Labeling of Nucleic Acids: Site-specific incorporation into oligonucleotides for EPR spectroscopy studies of DNA/RNA structure, dynamics, and conformational changes.
• Structural Biology Research: As a paramagnetic probe for measuring distances and mapping biomolecular interactions within complex nucleic acid-protein assemblies.
• Biophysical Assay Development: Used in the development of sensitive diagnostic assays and sensors that rely on changes in EPR signal upon target binding.
• Drug Discovery Screening: Employed in fragment-based drug discovery to study the binding of small molecules to RNA or DNA targets.
• Nucleic Acid Dynamics Studies: Investigation of local flexibility, backbone motions, and hybridization kinetics in model systems.
• Teaching & Advanced Reference Standard: Serves as a high-purity reference compound in academic and industrial research laboratories.

Basic Information

Product Name	3'-(2,2,5,5-Tetramethyl-3-Pyrroline-1-Oxyl-3-Carbonyl)Amino-3'-Deoxyguanosine 5'-Diphosphate
CAS No.	105802-04-8
Molecular Formula	C₂₀H₂₉N₈O₁₃P₂
Molecular Weight	642.44 g/mol
Synonyms	3'-SL-GDP; 3'-(TEMPO-Carbonyl)Amino-3'-deoxyGDP; 3'-Deoxy-3'-[[(2,2,5,5-tetramethyl-1-oxyl-3-pyrrolidinyl)carbonyl]amino]guanosine 5'-(trihydrogen diphosphate); Nitroxide-labeled GDP; 3'-TEMPO-amido-3'-dGDP; Spin-labeled guanosine diphosphate analog; 3'-Amino-3'-deoxyguanosine 5'-diphosphate, 3'-(2,2,5,5-tetramethyl-1-oxyl-3-pyrrolidinecarboxamide)
EINECS	Contact for details

Quality Control

Every batch of 3'-(2,2,5,5-Tetramethyl-3-Pyrroline-1-Oxyl-3-Carbonyl)Amino-3'-Deoxyguanosine 5'-Diphosphate is manufactured and tested under strict quality management systems. Our products undergo rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure compliance with research-grade specifications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment to guarantee traceability and consistency for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light should be avoided to maintain stability and purity. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Off-white to pale yellow solid
Identification (HPLC)	Conforms to reference standard
Identification (EPR)	Exhibits characteristic nitroxide radical signal
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90% (anhydrous basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.