Description

5-Azido-2'-Deoxyuridine 5'-Triphosphate is a chemically modified nucleotide triphosphate analog, specifically engineered for advanced biochemical research and diagnostic applications. Its core value lies in its azido functional group, which enables highly specific bioorthogonal click chemistry reactions for labeling and detection. This compound is essential for researchers and developers in the fields of molecular biology, nucleic acid chemistry, and next-generation sequencing technology development.

Application

• Click Chemistry Labeling: Acts as a key reagent for site-specific labeling of DNA via copper-catalyzed azide-alkyne cycloaddition (CuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC).
• Next-Generation Sequencing (NGS): Used in the development of novel sequencing-by-synthesis (SBS) platforms and single-molecule sequencing techniques that require cleavable or detectable terminator nucleotides.
• Probe Synthesis: Enables the creation of highly sensitive and specific fluorescent or affinity-tagged nucleic acid probes for FISH (Fluorescence In Situ Hybridization) and microarray applications.
• DNA Polymerase Studies: Serves as a substrate analog to investigate polymerase kinetics, fidelity, and mechanisms of nucleotide incorporation and excision.
• Bioconjugation & Drug Delivery: Facilitates the covalent attachment of drugs, reporter molecules, or nanoparticles to oligonucleotides for targeted therapeutic and diagnostic constructs.
• Crosslinking & Photaffinity Labeling: The azido group can be activated by UV light to form covalent crosslinks, useful for studying nucleic acid-protein interactions.

Basic Information

Item	Detail
Product Name	5-Azido-2'-Deoxyuridine 5'-Triphosphate
CAS No.	105449-09-0
Molecular Formula	C9H13N5O13P3
Molecular Weight	516.15 g/mol
Synonyms	5-Azido-dUTP; Azido-dUTP; 5-N3-dUTP; 5-Azido-2'-deoxyuridine-5'-triphosphate; 2'-Deoxy-5-azidouridine-5'-triphosphate; 5-Azido-2'-deoxyuridine triphosphate; dUTP-5-N3; 5-N3-2'-deoxyuridine-5'-triphosphate
EINECS	Contact for details

Quality Control

Every batch of 5-Azido-2'-Deoxyuridine 5'-Triphosphate is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure the highest standards of purity and performance for sensitive research applications. Our quality commitment includes comprehensive analysis via HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, and stability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to keep the material desiccated at -20°C. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5%
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.