Description

Dmt-Dc(Bz) Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer essential for automated oligonucleotide synthesis. This compound matters because it enables the precise and efficient incorporation of a cytidine derivative into synthetic DNA or RNA strands, ensuring high coupling efficiency and sequence fidelity. It is critically needed by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries for applications in antisense therapy, siRNA development, PCR primers, and molecular probes.

Application

• Oligonucleotide Synthesis: As a key building block (monomer) in solid-phase automated synthesizers for DNA/RNA.
• Therapeutic Oligonucleotide Production: Manufacturing of antisense oligonucleotides (ASOs), siRNA, and aptamers for drug development.
• Research & Diagnostic Probe Synthesis: Creation of labeled or unlabeled probes for PCR, sequencing, FISH, and microarray technologies.
• Primer Construction: Synthesis of specific primers for genetic testing, cloning, and amplification techniques.
• Synthesis of Modified Oligonucleotides: For introducing specific modifications to study structure-function relationships or enhance stability.
• Scale-up cGMP Manufacturing: Supporting the production of oligonucleotide-based Active Pharmaceutical Ingredients (APIs) under Good Manufacturing Practice (GMP) guidelines.

Basic Information

Item	Detail
Product Name	Dmt-Dc(Bz) Phosphoramidite
CAS No.	102212-98-6
Molecular Formula	C41H49N4O8P
Molecular Weight	756.83 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-benzoyl-2'-deoxycytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; DMT-dC(Bz) Phosphoramidite; N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine-3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); dC(Bz) Phosphoramidite; dCbz CE Phosphoramidite; 2'-Deoxycytidine, N4-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite)
EINECS	Contact for details

Quality Control

Our Dmt-Dc(Bz) Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for critical research and cGMP applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (¹H NMR, ³¹P NMR). A detailed Certificate of Analysis (COA) is provided with every shipment, documenting specifications for assay, moisture content (KF), and solvent residues.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.5%
Assay (³¹P NMR)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.