Description

β-Actin cDNA Probe from Mouse is a highly specific molecular biology reagent designed for the detection and quantification of β-actin mRNA expression. This probe is essential for normalizing gene expression data in techniques such as Northern blotting, RT-PCR, and in situ hybridization, ensuring accurate and reliable experimental results. It is a critical tool for researchers and diagnostic laboratories in the fields of molecular biology, genetics, oncology, and pharmaceutical development who require consistent and high-quality reference standards.

Application

• Gene Expression Normalization: Serves as an internal control (housekeeping gene) to normalize mRNA levels in quantitative RT-PCR (qPCR) and reverse transcription PCR (RT-PCR) assays.
• Northern Blot Analysis: Used as a hybridization probe to detect β-actin mRNA on Northern blots, confirming RNA integrity and loading consistency.
• In Situ Hybridization (ISH): Employed for the localization of β-actin mRNA within tissue sections or cell cultures in pathological and developmental studies.
• Diagnostic Assay Development: Integral component in the development and validation of molecular diagnostic kits for various diseases, including cancer research.
• Cell Biology Research: Used in studies of cytoskeleton dynamics, cell motility, and as a stable reference in experiments involving cellular stress or differentiation.
• Pharmaceutical Screening: Acts as a control in high-throughput screening assays to evaluate the effects of drug candidates on gene expression profiles.

Basic Information

Product Name	β-Actin cDNA Probe from Mouse
CAS No.	102055-33-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Actb Probe; β-Actin cDNA Probe; Mouse β-Actin Probe; Actin, Beta Probe; Housekeeping Gene Probe; ACTB cDNA; Reference Gene Probe; Normalization Probe
EINECS	Contact for details

Quality Control

Our molecular biology reagents, including the β-Actin cDNA Probe, are produced and tested under stringent quality management systems. Each batch is validated for specificity, sensitivity, and lack of contaminating nucleases through rigorous analytical methods. A comprehensive Certificate of Analysis (COA), detailing purity, concentration, and functional performance data, is provided to ensure traceability and reliability for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. Handle under controlled conditions to minimize the risk of degradation.

Specification

Item	Specification
Appearance	Clear, colorless solution
Concentration	As per COA (Typically 25-100 ng/µL)
Purity (A260/A280 Ratio)	1.8 - 2.0
Specificity	Hybridizes specifically to mouse β-actin mRNA
Performance (qPCR)	Efficiency: 90-110%; Single peak in melt curve analysis
Nuclease Contamination	Not detected
Buffer	10 mM Tris-HCl, 1 mM EDTA, pH 8.0

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.