Description

Restriction Endonuclease Apal I From*Ace Tobacter Pa is a highly specific enzyme that recognizes and cleaves DNA at the palindromic sequence 5'-GGGCCC-3'. This precision is critical for molecular biology applications requiring accurate DNA manipulation and analysis. It is an essential tool for researchers and professionals in biotechnology, pharmaceutical development, and academic research laboratories engaged in genetic engineering, cloning, and genomic mapping.

Application

• Molecular Cloning: Essential for the precise cutting and insertion of DNA fragments into vectors.
• Genetic Engineering: Used in the construction of recombinant DNA molecules for research and therapeutic protein production.
• Genomic Mapping: Aids in the analysis of genome structure and the identification of genetic markers.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: Key enzyme for detecting DNA sequence variations in genetic diagnostics and forensics.
• DNA Sequencing Library Preparation: Facilitates the fragmentation and preparation of DNA for next-generation sequencing platforms.
• Diagnostic Assay Development: Used in the design of tests for specific genetic disorders or pathogen detection.

Basic Information

Product Name	Restriction Endonuclease Apal I From*Ace Tobacter Pa
CAS No.	100630-51-1
Molecular Formula	Protein
Molecular Weight	Contact for details
Synonyms	ApaLI; Apal I Restriction Enzyme; Endodeoxyribonuclease Apal I; Restriction Endonuclease ApaLI; EC 3.1.21.4; DNA Apal I methyltransferase; Type II Site-Specific Deoxyribonuclease Apal I
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Apal I is produced and tested under stringent quality management systems. Each lot undergoes rigorous analytical testing to ensure high specific activity, purity, and the absence of contaminating nucleases. Certificates of Analysis (COA) detailing activity units, purity assessments, and functional testing in standard buffers are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a storage buffer containing 50% glycerol to prevent freezing at -20°C.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves lambda DNA at the specific recognition site (5'-GGGCCC-3')
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 90% single band
Exonuclease Activity	Not detectable
Endonuclease Activity	Not detectable in standard assay
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.