Description

Deoxyribonucleic Acid, Col E1 Plasmid, Solution is a highly purified, ready-to-use solution containing the ColE1 plasmid DNA, a fundamental tool in molecular biology and genetic engineering. This product is essential for researchers and developers requiring a reliable, standardized source of plasmid DNA for cloning, transfection, and gene expression studies. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical companies engaged in recombinant DNA technology, vaccine development, and therapeutic protein production.

Application

• Molecular Cloning: Serves as a standard vector for inserting and amplifying foreign DNA fragments in bacterial hosts like E. coli.
• Transfection & Gene Expression Studies: Used to introduce genetic material into eukaryotic cells to study gene function and protein production.
• Reference Standard: Acts as a control or calibration standard in quantitative PCR (qPCR), sequencing, and other nucleic acid analysis techniques.
• Recombinant Protein Production: Provides the genetic blueprint for the expression of target proteins in engineered cell systems.
• Vaccine & Therapeutic Development: Utilized in the research and development of DNA vaccines and gene therapies.
• Biotechnology Education & Training: A key reagent for teaching core techniques in genetic engineering and molecular biology laboratories.

Basic Information

Product Name	Deoxyribonucleic Acid, Col E1 Plasmid, Solution
CAS No.	100209-25-4
Molecular Formula	N/A (Biopolymer)
Molecular Weight	Variable (Plasmid-dependent)
Synonyms	ColE1 Plasmid DNA; pColE1; Colicin E1 Plasmid; Plasmid ColE1 DNA Solution; ColE1 Vector; Bacterial Plasmid DNA; Supercoiled DNA; Covalently Closed Circular DNA (cccDNA); Recombinant Plasmid DNA
EINECS	Contact for details

Quality Control

Our ColE1 Plasmid DNA Solution is manufactured under stringent conditions to ensure batch-to-batch consistency and high performance. Quality is verified through a series of analytical tests including agarose gel electrophoresis for topology and purity, spectrophotometry for concentration and purity (A260/A280 ratio), and restriction analysis for identity. Certificates of Analysis (COA) detailing purity, concentration, endotoxin levels, and sterility are provided with each batch to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C ± 5°C. Avoid repeated freeze-thaw cycles. Under these conditions, the product is typically stable for at least 24 months.

Specification

Item	Specification
Appearance	Clear, colorless to slightly opalescent solution
Concentration	0.1 - 1.0 mg/mL (Lot-specific)
Purity (A260/A280)	1.8 - 2.0
Topology	>90% Supercoiled (cccDNA)
Identification (Gel Electrophoresis)	Conforms to standard
Endotoxin Level	< 10 EU/mg DNA
Sterility (Membrane Filtration)	Sterile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.