Description

5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide Cyclohexylammonium Salt is a high-purity chromogenic substrate specifically designed for enzymatic detection. This compound is critical for the sensitive and specific visualization of β-glucuronidase activity, a widely used reporter system in molecular biology and diagnostics. It is an essential reagent for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries, enabling applications from gene expression studies to microbial detection.

Application

• Molecular Biology Research: As a chromogenic substrate for detecting β-glucuronidase (GUS) reporter gene expression in plant, bacterial, and mammalian cell systems.
• Microbiological Testing: For the specific identification and enumeration of E. coli and other coliform bacteria in water, food, and environmental samples using selective media (e.g., in MI Agar, X-Gluc based assays).
• Diagnostic Kit Manufacturing: A key raw material in the production of diagnostic kits and strips for urinary tract infections and other bacterial assays.
• Histochemistry & Cell Biology: Used for in situ localization of GUS enzyme activity in tissue sections and fixed cells, providing spatial information on gene expression.
• Pharmaceutical Quality Control: Employed in sterility testing and microbial limit tests within GMP environments to detect microbial contamination.
• Enzyme Activity Assays: For the quantitative and qualitative measurement of β-glucuronidase enzyme activity in research and industrial enzyme preparations.

Basic Information

Item	Detail
Product Name	5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide Cyclohexylammonium Salt
CAS No.	18656-96-7
Molecular Formula	C20H26BrClN2O7
Molecular Weight	521.79 g/mol
Synonyms	X-Glucuronide, Cyclohexylammonium Salt; 5-Bromo-4-chloro-3-indolyl-β-D-glucopyranosiduronic acid cyclohexylammonium salt; BCIG; X-β-D-Glucuronide; 5-Bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid cyclohexylammonium salt; GUS Substrate; β-Glucuronidase Substrate
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide Cyclohexylammonium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing purity, assay, and related substances are provided with every shipment. We support compliance with relevant industry standards for raw materials used in diagnostic and research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term storage, consider desiccant and under inert atmosphere to maintain optimal stability and performance. Keep container tightly sealed when not in use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	< 20 ppm
Biological Activity	Yields intense blue precipitate upon enzymatic cleavage

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.