Description

Superose 6 Prep Grade is a high-performance, cross-linked agarose-based chromatography resin designed for preparative-scale size-exclusion chromatography (SEC). This product is engineered for the high-resolution separation and purification of biomolecules, particularly proteins, peptides, and nucleic acids, in the 5,000 to 5,000,000 molecular weight range. It is an essential tool for research laboratories, biopharmaceutical development, and production facilities requiring reliable, scalable purification processes.

Application

• Preparative Protein Purification: Ideal for the final polishing step in the purification of monoclonal antibodies, recombinant proteins, and enzymes.
• Desalting and Buffer Exchange: Efficient removal of salts, detergents, or other small molecules from biological samples.
• Oligonucleotide and Plasmid DNA Separation: Used for the purification and analysis of nucleic acids based on size.
• Virus and Vaccine Purification: Applicable in the downstream processing of viral vectors and vaccines.
• Aggregate Removal: Critical for separating high molecular weight aggregates from monomeric protein formulations in quality control.
• Process Development and Scale-Up: Serves as a reliable media for optimizing and scaling chromatographic processes from lab to pilot and production scale.

Basic Information

Product Name	Superose 6 Prep Grade
CAS No.	98726-62-6
Molecular Formula	Cross-linked agarose polymer
Molecular Weight	Not applicable (polymeric resin)
Synonyms	Superose 6; Superose™ 6 Prep Grade; Agarose 6B; Cross-linked beaded agarose; Size exclusion chromatography resin; SEC resin; Gel filtration medium; Pharmacia Superose 6
EINECS	Contact for details

Quality Control

Our Superose 6 Prep Grade is manufactured under a quality management system and undergoes rigorous testing to ensure batch-to-batch consistency and performance. Key parameters such as particle size distribution, flow rate, pressure tolerance, and exclusion limits are strictly controlled. A Certificate of Analysis (COA) detailing the specifications for each lot is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (36-46°F) in 20% ethanol or an appropriate storage solution as specified. Do not allow the resin to freeze or dry out. The product is hygroscopic (moisture-sensitive); ensure the container is sealed after use to prevent moisture absorption and microbial growth.

Specification

Item	Specification
Appearance	White suspension
Matrix	Cross-linked 6% agarose beads
Particle Size	20-40 μm (mean)
Exclusion Limit (Globular Proteins)	~5 x 10⁶ Da
Operating pH Range	3 - 12 (long term); 2 - 14 (short term)
Maximum Operating Pressure	0.3 MPa (3 bar, 44 psi)
Flow Rate (Recommended)	30 - 90 cm/h
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.