Description

Methidiumspermine Sepharose is a specialized affinity chromatography medium designed for the purification of nucleic acid-binding proteins and enzymes. This product is critical for researchers and process developers requiring high selectivity and binding capacity in downstream bioprocessing. It is essential for applications in molecular biology, proteomics, and the production of therapeutic biologics, serving pharmaceutical, biotechnology, and academic research sectors globally.

Application

• Affinity Purification of DNA/RNA-Binding Proteins: Isolation of transcription factors, polymerases, nucleases, and other nucleic acid-interacting proteins from complex biological mixtures.
• Enzyme Activity Studies: Immobilization of nucleic acids or proteins to study enzyme kinetics, binding affinities, and molecular interactions.
• Proteomics Research: Capture and identification of protein complexes involved in gene regulation and DNA repair pathways.
• Process Development for Biologics: A key tool in developing scalable, cGMP-compliant purification processes for therapeutic proteins.
• High-Throughput Screening (HTS): Used in automated platforms for drug discovery targeting nucleic acid-protein interactions.
• Diagnostic Assay Development: Component in diagnostic kits for detecting specific DNA-binding antibodies or proteins in clinical samples.

Basic Information

Product Name	Methidiumspermine Sepharose
CAS No.	123757-91-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Methidium-spermine Sepharose; Sepharose-Methidiumspermine; Methidium spermine coupled to Sepharose; Affinity gel for DNA-binding proteins; Methidiumspermine-agarose; DNA-affinity chromatography medium; MSP-Sepharose; Nucleic acid affinity resin
EINECS	Contact for details

Quality Control

Our Methidiumspermine Sepharose is manufactured under a strict quality management system. Each batch undergoes rigorous testing for ligand density, binding capacity, flow properties, and absence of microbial contamination to ensure performance consistency and reliability. Certificates of Analysis (COA) detailing all specifications are available upon request. Production adheres to guidelines suitable for research and process development use.

Storage

Preserve in a tightly closed container, protected from light. Store suspended in the recommended storage solution (typically 20% ethanol) at 2-8°C. Do not freeze. Keep the container sealed to prevent evaporation and microbial growth. For long-term stability, store under the conditions specified on the product label or Certificate of Analysis.

Specification

Item	Specification
Appearance	Slurry in storage solution
Matrix	Cross-linked 4% or 6% agarose beads
Ligand	Methidiumspermine
Ligand Density	Contact for details
Binding Capacity	Contact for details
Particle Size	45-165 μm (wet)
Maximum Flow Rate	Contact for details
pH Stability	3 - 10 (long term), 2 - 12 (short term)
Storage Solution	20% Ethanol, 1 mM EDTA (typical)
Microbial Bioburden	< 10 CFU/mL

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.