Description

Sepharose Cl-4B is a beaded, cross-linked agarose gel widely used as a high-performance matrix for gel filtration chromatography. Its value lies in providing excellent resolution for the separation and purification of large biomolecules, such as proteins, nucleic acids, and viruses, based on their size. This product is essential for research laboratories, biopharmaceutical manufacturers, and diagnostic kit producers requiring reliable, large-pore chromatography media for downstream processing and analytical applications.

Application

• Size-Exclusion Chromatography (SEC/GFC): Primary application for the separation and purification of macromolecules like antibodies, enzymes, plasmids, and polysaccharides.
• Virus and Vaccine Purification: Effective for the size-based isolation and purification of viral particles and vaccines in bioprocessing.
• Desalting and Buffer Exchange: Rapid removal of salts, dyes, or other small molecules from protein and nucleic acid samples.
• Molecular Weight Estimation: Used to determine the approximate molecular weight of unknown proteins or polymers by comparison with standard curves.
• Oligonucleotide and Plasmid DNA Purification: Separation of supercoiled plasmid DNA from genomic DNA, RNA, and proteins, or purification of synthetic oligonucleotides.
• Aggregate Analysis: Detection and quantification of high molecular weight aggregates in therapeutic protein formulations.
• Cell Lysate Fractionation: Initial fractionation step to remove large cellular debris and isolate soluble proteins of interest.
• Coupling Support: The activated matrix can serve as a support for affinity ligand immobilization (e.g., for antibody purification).

Basic Information

Product Name	Sepharose Cl-4B
CAS No.	61970-08-9
Molecular Formula	(C12H18O9)n
Molecular Weight	High Polymer
Synonyms	Agarose, cross-linked; Sepharose 4B-CL; Sepharose CL-4B; CL-Sepharose 4B; Cross-linked Sepharose 4B; Agarose Beads 4%; 4% Cross-linked Agarose; Agarose Gel, beaded; Chromatography Media, Agarose-based; Gel Filtration Medium
EINECS	Contact for details

Quality Control

Our Sepharose Cl-4B is produced under a strict quality management system. Each batch is tested to ensure consistent performance characteristics, including bead size distribution, exclusion limits, flow properties, and chemical stability. Certificates of Analysis (COA) detailing these specifications are available upon request to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (36-46°F) in its supplied storage solution (typically containing a preservative) to prevent microbial growth and maintain chromatographic performance. Do not freeze. This product is hygroscopic (moisture-sensitive); ensure the container is properly sealed after use to prevent dehydration or contamination.

Specification

Item	Specification
Appearance	White, opaque beads in suspension
Matrix	Cross-linked 4% Agarose
Bead Size Range	45-165 μm
Exclusion Limit (Globular Proteins)	~20,000 kDa
Operating pH Range	3 - 13 (long term), 2 - 14 (short term)
Chemical Stability	Stable in most aqueous buffers, detergents, and 8M urea. Not stable in oxidizing agents.
Maximum Linear Flow Rate	> 30 cm/h
Maximum Pressure	0.1 MPa, 15 psi

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.