Description

Bio-Gel P-4 is a high-performance, beaded polyacrylamide gel filtration medium designed for the separation of biomolecules in aqueous solutions. This product is essential for researchers and process engineers requiring precise fractionation of peptides, oligonucleotides, and other small biomolecules in the 800 to 4,000 Daltons range. It is a critical tool for laboratories and production facilities in the biotechnology, pharmaceutical, and life science research sectors.

Application

• Desalting and Buffer Exchange: Rapid removal of salts, dyes, or other small molecules from protein, peptide, and nucleic acid samples.
• Fractionation of Biomolecules: Size-based separation of peptides, oligonucleotides, small proteins, and polysaccharides.
• Sample Purification: Clean-up steps in enzymatic reaction preparations, DNA sequencing, and peptide synthesis workflows.
• Analytical Chromatography: Used in low-pressure liquid chromatography systems for molecular weight estimation and purity analysis.
• Biopharmaceutical Downstream Processing: Intermediate purification steps for therapeutic peptides and oligonucleotides.
• Laboratory-Scale Process Development: Method scouting and optimization for scalable biomolecule purification processes.

Basic Information

Product Name	Bio-Gel P-4
CAS No.	39454-59-6
Molecular Formula	(C3H5NO)n
Molecular Weight	Exclusion limit ~4,000 Daltons (bead form)
Synonyms	Polyacrylamide Gel P-4; Bio-Gel P4; Polyacrylamide Beads, Bio-Gel P-4; Gel Filtration Medium P-4; Bio-Rad Bio-Gel P-4; Polyacrylamide Chromatography Medium; Acrylamide Polymer Gel; Bio-Gel P-4 Fine
EINECS	Contact for details

Quality Control

Our Bio-Gel P-4 is manufactured under controlled conditions to ensure batch-to-batch consistency for critical separations. Each lot undergoes rigorous quality testing, including evaluation of particle size distribution, exclusion limits, flow characteristics, and chemical stability. Certificates of Analysis (COA) detailing these specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). The product is supplied as a suspension; do not allow the beads to dry out. Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	White fine beads, aqueous suspension
Exclusion Limit (Globular Proteins)	~4,000 Daltons
Fractionation Range (Globular Proteins)	800 - 4,000 Daltons
Bed Volume	~5 mL/g (swollen)
Operating pH Range	2 - 10
Maximum Linear Flow Rate	Contact for details
Particle Size	Fine grade (specific range available on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.