Description

Sephadex G-75 is a cross-linked dextran gel designed for size-exclusion chromatography (SEC), also known as gel filtration. This product is essential for the separation and purification of biomolecules, particularly peptides and small proteins, based on their hydrodynamic volume. It is a critical tool for researchers and process engineers in the biotechnology, pharmaceutical, and life science industries requiring high-resolution fractionation. The compound is identified by CAS No. 37224-29-6.

Application

• Biomolecule Purification: Desalting, buffer exchange, and group separation of peptides, oligonucleotides, and small proteins from larger molecules.
• Pharmaceutical Development: Critical step in downstream processing for the purification of therapeutic peptides and protein fragments.
• Analytical Chromatography: Used in laboratory-scale SEC for molecular weight estimation and analysis of complex biological mixtures.
• Enzyme Preparation: Removal of low molecular weight contaminants and salts from enzyme preparations.
• Sample Preparation for HPLC/MS: Clean-up step to remove interfering salts and detergents prior to mass spectrometric analysis.
• Industrial Bioprocessing: Scalable purification step in the manufacture of fine chemicals and biologics.

Basic Information

Product Name	Sephadex G-75
CAS No.	37224-29-6
Molecular Formula	(C6H10O5)n
Molecular Weight	High polymer
Synonyms	Dextran gel G-75; Sephadex Gel Filtration Medium G-75; Cross-linked dextran; Gel filtration grade dextran; Size exclusion chromatography dextran; G-75 Beaded Dextran; Pharmacia Sephadex G-75; Dextran cross-linked with epichlorohydrin
EINECS	Contact for details

Quality Control

Our Sephadex G-75 is manufactured and tested under a rigorous quality management system. Each batch is characterized for critical parameters including particle size distribution, exclusion limits, and swelling properties to ensure consistent chromatographic performance. Certificates of Analysis (COA) detailing these specifications are available upon request to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent moisture uptake, which can affect swelling and performance.

Specification

Item	Specification
Appearance	White to off-white free-flowing beads
Exclusion Limit (Globular Proteins)	~3,000 - 80,000 Da
Bed Volume (swollen in water)	12-15 mL/g
Particle Size (dry)	40-120 μm
Water Content	Contact for details
Ash Content	< 0.5 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.