Description

Affi-Gel 10 is a pre-activated N-hydroxysuccinimide (NHS) ester agarose resin designed for the covalent immobilization of primary amine-containing ligands, such as proteins, peptides, and antibodies. This product matters because it enables rapid, efficient, and stable coupling under mild conditions, forming amide bonds without the need for carbodiimide activation. It is an essential tool for researchers and process developers in biopharmaceutical purification, affinity chromatography, and diagnostic assay development who require high-capacity, low non-specific binding matrices for capturing target biomolecules.

Application

• Affinity Chromatography Media: For the purification of antibodies, recombinant proteins, enzymes, and other biomolecules.
• Immunoassay Development: Immobilization of antigens or antibodies for ELISA, lateral flow assays, and biosensor surfaces.
• Proteomics and Protein Research: Creation of custom affinity columns for studying protein-protein interactions and pull-down assays.
• Biopharmaceutical Downstream Processing: Scalable ligand coupling for manufacturing-grade affinity purification steps.
• Diagnostic Kit Manufacturing: Production of stable, high-performance solid phases for clinical diagnostic tests.
• Enzyme Immobilization: Support for biocatalysts used in synthesis and industrial bioprocesses.

Basic Information

Item	Details
Product Name	Affi-Gel 10
CAS No.	60454-66-2
Molecular Formula	N/A (Functionalized cross-linked agarose bead)
Molecular Weight	N/A (Matrix-based product)
Synonyms	N-Hydroxysuccinimide-activated Agarose; NHS-Activated Agarose; Affi-Gel® 10; N-Succinimidyl Agarose; Activated CH Sepharose 4B analogue; 6-Aminocaproic acid N-hydroxysuccinimide ester agarose; Carboxyhexyl-agarose active ester
EINECS	Contact for details

Quality Control

Our Affi-Gel 10 is manufactured under strict quality systems. Each lot is tested to ensure consistent performance, including parameters for ligand coupling capacity, bead size distribution, and functional group stability. Certificates of Analysis (COA) detailing these specifications are provided with every shipment to guarantee the material meets the rigorous demands of research and process development.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is supplied as a suspension in isopropanol. Avoid freezing and prolonged exposure to moisture. Keep the container sealed to prevent solvent evaporation and hydrolysis of the active ester groups.

Specification

Item	Specification
Matrix	Cross-linked 4% Agarose Beads
Active Group	N-Hydroxysuccinimide (NHS) Ester
Coupling Chemistry	Amine-reactive
Ligand Coupling Capacity	> 15 µmol NHS ester per mL drained gel
Bead Size Range	45 - 165 µm
Exclusion Limit	> 2 x 10⁷ Daltons
Storage Solvent	Isopropanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.