Description

Sepharose Cl-6B is a beaded, cross-linked agarose gel designed for high-performance gel filtration chromatography. This product is essential for the size-based separation and purification of large biomolecules, including proteins, nucleic acids, and viruses, in both research and industrial-scale processes. It is a critical tool for biopharmaceutical manufacturers, academic research laboratories, and diagnostic kit producers requiring reliable, high-capacity separation media.

Application

• Size-exclusion chromatography (SEC) for the purification and analysis of macromolecules.
• Desalting and buffer exchange of protein and nucleic acid samples.
• Fractionation of serum proteins, monoclonal antibodies, and other therapeutic biologics.
• Separation of plasmid DNA, RNA, and large protein complexes.
• Virus purification and analysis in vaccine development.
• Removal of aggregates from final drug product formulations.
• As a support matrix for the immobilization of ligands in affinity chromatography.
• Process development and scale-up in downstream bioprocessing.

Basic Information

Product Name	Sepharose Cl-6B
CAS No.	62610-50-8
Molecular Formula	(C12H18O9)n
Molecular Weight	High molecular weight polymer
Synonyms	Agarose 6B, CL-6B; Sepharose 6B Cross-linked; Cross-linked Agarose 6B; 6% Cross-linked Agarose; Gel Filtration Medium 6B; Agarose Gel, Cross-linked; CL-Sepharose 6B; Agarose 6B CL
EINECS	Contact for details

Quality Control

Our Sepharose Cl-6B is manufactured under strict quality systems to ensure batch-to-batch consistency for critical separations. Each lot is tested for key performance parameters including bead size distribution, exclusion limits, flow properties, and chemical stability. Certificates of Analysis (COA) detailing these specifications are provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in 20% ethanol or an appropriate preservative solution as supplied. Do not freeze. Keep away from strong oxidizing agents and extremes of pH.

Specification

Item	Specification
Matrix	Cross-linked 6% Agarose
Bead Size	45-165 μm
Exclusion Limit (Globular Proteins)	~4 x 10^6 Da
Operating pH Range	2 - 14 (short term), 3 - 12 (long term)
Maximum Linear Flow Rate	15-30 cm/h
Maximum Pressure	0.1 MPa, 0.8 bar
Storage Temperature	2-8°C (in preservative)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.