Description

Sepharose 6Mb is a high-performance, beaded agarose-based chromatography medium designed for size exclusion and affinity purification of large biomolecules. Its value lies in its exceptional mechanical stability and high flow rates, enabling efficient, scalable separations in industrial bioprocessing. This product is essential for researchers and manufacturers in the biotechnology and pharmaceutical industries requiring robust purification of viruses, plasmids, proteins, and other macromolecular complexes.

Application

• Virus Purification and Concentration: Ideal for downstream processing of viral vectors for gene therapy and vaccine production, including adenovirus, AAV, and lentivirus.
• Plasmid DNA (pDNA) Isolation: Used in the final polishing steps to separate supercoiled plasmid DNA from open circular and linear forms, as well as RNA and endotoxins.
• Large Protein Complex and Aggregate Analysis: Effective for the size-based separation of antibodies, antibody-drug conjugates (ADCs), and other high molecular weight protein aggregates.
• Affinity Chromatography Matrix: Serves as a robust base matrix for the immobilization of ligands, such as proteins A or G, for antibody purification.
• Cell Debris and Whole Cell Removal: Acts as a depth filter or pre-column step in clarification processes for crude fermentation and cell culture broths.
• Process Development and Scale-Up: Provides a scalable platform from laboratory research to commercial manufacturing due to its consistent particle size and flow properties.

Basic Information

Product Name	Sepharose 6Mb
CAS No.	64296-53-3
Molecular Formula	(C12H18O9)n
Molecular Weight	High molecular weight polymer
Synonyms	Agarose 6MB; Cross-linked Sepharose 6MB; Sepharose 6 Macro Beads; Agarose Beads, 6% cross-linked; 6% Cross-linked Agarose; Macroporous Agarose Gel; Chromatography Medium, Agarose-based; Size Exclusion Chromatography Media
EINECS	Contact for details

Quality Control

Our Sepharose 6Mb is manufactured under a quality management system. Each lot is tested to ensure consistent performance characteristics critical for chromatography, including bead size distribution, exclusion limits, flow rate, and pressure-flow relationships. Certificates of Analysis (COA) detailing these specifications are available upon request to support your regulatory and process documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in 20% ethanol or an appropriate storage solution as specified. Do not freeze. Keep the suspension moist at all times to prevent drying and cracking of the beads.

Specification

Item	Specification
Matrix	Cross-linked 6% Agarose
Bead Size (mean)	80 - 160 μm
Exclusion Limit (Proteins)	> 4 x 10^7 Da
Maximum Linear Flow Rate	> 300 cm/h
Operating Pressure	> 0.3 MPa
pH Stability (long term)	3 - 12
Chemical Stability	Stable in most aqueous buffers, detergents, and 8M urea. Avoid strong oxidizing agents.
Storage Solution	20% Ethanol in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.