Description

Sephadex G-200 is a high-performance, cross-linked dextran gel designed for size-exclusion chromatography (SEC) and gel filtration. This product is essential for the precise separation and purification of large biomolecules, such as proteins, polysaccharides, and nucleic acids, based on their hydrodynamic volume. It is a critical tool for researchers and process engineers in the biotechnology, pharmaceutical, and life science industries requiring high-resolution fractionation for analysis or downstream processing. Sephadex G-200 CAS NO 9041-36-5 is known for its excellent chemical stability and reproducible performance.

Application

• Biomolecule Purification: Desalting, buffer exchange, and group separation of proteins, peptides, and enzymes.
• Molecular Weight Determination: Estimation of molecular weights of proteins and other macromolecules via gel filtration calibration.
• Pharmaceutical Development: Purification steps in the production of vaccines, antibodies, and other biologics.
• Life Science Research: Fractionation of cell lysates, isolation of specific nucleic acid fragments, and sample preparation for analytical techniques.
• Industrial Enzyme Processing: Downstream purification of industrial enzymes from fermentation broths.
• Polysaccharide Analysis: Separation and analysis of dextrans, starches, and other complex carbohydrates.

Basic Information

Product Name	Sephadex G-200
CAS No.	9041-36-5
Molecular Formula	(C6H10O5)n
Molecular Weight	High polymer (Variable)
Synonyms	Dextran gel; Cross-linked dextran; Gel filtration medium G-200; Sephadex® G-200; Size exclusion chromatography gel; Gel chromatography medium; Polydextran gel; Gel filtration matrix
EINECS	Contact for details

Quality Control

Our Sephadex G-200 is produced and tested under a rigorous quality management system. Each batch is characterized for critical parameters including particle size distribution, exclusion limits, swelling volume, and flow properties to ensure consistent chromatographic performance and lot-to-lot reproducibility. While not a simple chemical compound with a defined purity, specifications are strictly controlled. Certificates of Analysis (COA) with detailed performance data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive). Ensure the container is securely sealed after each use to prevent moisture uptake, which can affect swelling characteristics and performance. For long-term storage, consider using a desiccant.

Specification

Item	Specification
Product Form	Dry, free-flowing beads
Exclusion Limit (Globular Proteins)	~200,000 Da
Fractionation Range (Globular Proteins)	5,000 - 800,000 Da
Bed Volume (Swollen in water)	Contact for details
Particle Size (Dry)	40 - 120 μm (Fine)
Chemical Stability	Stable in water, salt solutions, and alkaline media. Unstable in strong oxidizing agents.

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.