Description

Sephadex is a cross-linked dextran gel widely used as a molecular sieve for size-exclusion chromatography (SEC) and gel filtration. Its consistent bead size and high chemical stability make it a critical tool for the separation and purification of biomolecules, peptides, and polysaccharides. This product is essential for researchers and process engineers in the pharmaceutical, biotechnology, and life science industries who require reliable, high-resolution separation media for downstream processing and analytical applications.

Application

• Size-Exclusion Chromatography (SEC/GFC): For the separation and purification of proteins, enzymes, antibodies, nucleic acids, and other biomolecules based on molecular size.
• Desalting and Buffer Exchange: Rapid removal of salts, dyes, or other small molecules from protein and nucleic acid samples.
• Molecular Weight Determination: Used in analytical SEC to estimate the molecular weight of unknown proteins and polymers by comparison with standard curves.
• Sample Preparation: Clarification and group separation of complex biological mixtures prior to further analysis (e.g., HPLC, MS).
• Pharmaceutical Purification: Downstream processing step in the manufacture of therapeutic proteins, vaccines, and other biologics.
• Laboratory-Scale Fractionation: A staple tool in research laboratories for purifying specific components from cell lysates, culture media, or reaction mixtures.

Basic Information

Product Name	Sephadex
CAS No.	9014-76-0
Molecular Formula	(C6H10O5)n
Molecular Weight	High polymer (varies by grade)
Synonyms	Dextran gel; Cross-linked dextran; Gel filtration dextran; Size exclusion dextran gel; Sephadex G-type; Dextran bead; Chromatography media; Molecular sieve dextran; Gel chromatography matrix
EINECS	Contact for details

Quality Control

Our Sephadex is manufactured under strict quality systems to ensure batch-to-batch consistency critical for chromatographic performance. Each lot is tested for key parameters including bead size distribution, exclusion limits, swelling volume, and flow properties. A Certificate of Analysis (COA) detailing these specifications is provided with every shipment. We support applications requiring adherence to cGMP and ISO 9001 quality standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent moisture uptake, which can affect swelling properties and performance.

Specification

Item	Specification
Appearance	White to off-white free-flowing beads
Bead Size Range	20-150 μm (Grade dependent)
Exclusion Limit (MW)	Grade dependent (e.g., G-10 to G-200)
Swelling Volume	4-40 mL/g dry gel (Grade dependent)
pH Stability Range	2 - 13
Chemical Stability	Stable in water, salt solutions, and organic solvents
Moisture Content	< 10%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.