Description

Toyopearl 650 is a high-performance, cross-linked polymethacrylate-based chromatography resin designed for size exclusion, hydrophobic interaction, and ion exchange separations. This versatile medium is critical for achieving high-resolution purification of biomolecules, including proteins, peptides, and nucleic acids, ensuring product purity and process scalability. It is an essential tool for downstream processing in the biopharmaceutical, biotechnology, and life sciences research sectors.

Application

• Biopharmaceutical Purification: Final polishing and intermediate purification steps for monoclonal antibodies (mAbs), recombinant proteins, vaccines, and other therapeutic biologics.
• Process Development & Scale-Up: Used in laboratory-scale process optimization and seamlessly scalable to pilot and industrial manufacturing columns.
• Size Exclusion Chromatography (SEC): For desalting, buffer exchange, and fractionation of biomolecules based on hydrodynamic size.
• Hydrophobic Interaction Chromatography (HIC): Separation of proteins based on surface hydrophobicity, often used for removing aggregates and fragments.
• Ion Exchange Chromatography (IEX): Capture and purification of target molecules based on charge differences, utilizing the resin's derivatizable surface.
• Diagnostic & Analytical Applications: Purification of enzymes, antibodies, and other reagents used in diagnostic kits and analytical assays.
• Academic & Industrial Research: A reliable medium for protein characterization, complex sample analysis, and method development in R&D laboratories.

Basic Information

Product Name	Toyopearl 650
CAS No.	86159-29-7
Molecular Formula	(C5H8O2)n • (C4H6O2)m
Molecular Weight	N/A (Polymeric Resin)
Synonyms	Poly(ethylene glycol methacrylate-co-methyl methacrylate)
PEGMA-MMA copolymer
Hydrophilic polymethacrylate resin
TSKgel Toyopearl HW-65 (similar base matrix)
Chromatography medium 650
Polyhydroxyethyl methacrylate-based resin (related)
Cross-linked polymethacrylate bead
EINECS	Contact for details

Quality Control

Our Toyopearl 650 is manufactured under a strict quality management system. Each lot is tested to ensure consistent performance characteristics, including particle size distribution, porosity, pressure-flow properties, and chemical stability. Certificates of Analysis (COA) detailing lot-specific data are available upon request. Production can adhere to relevant GMP and ISO 9001 guidelines for applications requiring regulatory compliance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). The resin should be kept in the supplied storage solution (typically 20% ethanol) to prevent microbial growth and maintain performance. Avoid freezing.

Specification

Item	Specification
Matrix	Cross-linked Polymethacrylate
Particle Size	Contact for details
Particle Shape	Spherical
Exclusion Limit (Globular Protein)	Contact for details
Operating Pressure	> 0.3 MPa
pH Stability	2 - 13
Chemical Stability	Stable in most aqueous buffers, alcohols, and polar organic solvents.
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.