Description

Toyopearl Hw-65F is a high-performance, hydrophobic interaction chromatography (HIC) resin designed for the purification of biomolecules. This product is critical for achieving high-resolution separation of proteins, peptides, and nucleic acids based on their surface hydrophobicity. It is an essential tool for downstream processing in the biopharmaceutical, life sciences research, and diagnostic reagent manufacturing industries.

Application

• Purification of monoclonal antibodies (mAbs) and antibody fragments from complex cell culture harvests.
• Separation of recombinant proteins and enzymes in biomanufacturing processes.
• Polishing step for the removal of aggregates, host cell proteins, and process-related impurities.
• Fractionation of plasma proteins such as immunoglobulins and coagulation factors.
• Research-scale purification of hydrophobic peptides and membrane proteins in academic and industrial labs.
• Viral vector and vaccine purification in advanced therapy medicinal product (ATMP) production.

Basic Information

Product Name	Toyopearl Hw-65F
CAS No.	84932-18-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Toyopearl HW-65F; TOSOH HW-65F; Hydrophobic Interaction Chromatography Resin HW-65F; Polyhydroxymethyl methacrylate resin; HIC Media HW-65F; 84932-18-3; Hydrophobic Toyopearl
EINECS	Contact for details

Quality Control

Our Toyopearl Hw-65F is manufactured under a strict quality management system. Each lot is tested to ensure consistent performance characteristics, including particle size distribution, ligand density, and dynamic binding capacity. A Certificate of Analysis (COA) detailing these specifications is provided with every shipment to guarantee compliance with your process requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). Do not allow the resin to freeze. Keep the storage container sealed to prevent microbial contamination and maintain the integrity of the storage solution (typically 20% ethanol).

Specification

Item	Specification
Matrix	Hydrophilic methacrylate polymer
Particle Size	65 μm (Fine grade)
Ligand	Butyl group
Mean Particle Size	65 ± 10 μm
Dynamic Binding Capacity (for BSA)	> 25 mg/mL
pH Stability	2 - 13 (long term), 1 - 14 (short term)
Maximum Pressure	0.3 MPa
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.