Description

Tris(Carboxymethyl)Ethylenediamine-Sepharose is a specialized affinity chromatography medium designed for the purification of proteins and enzymes. This product is critical for achieving high-purity separations in research and bioprocessing applications. It is essential for laboratories and production facilities in the biotechnology, pharmaceutical, and life sciences sectors requiring robust and reliable purification tools.

Application

• Affinity Chromatography: Primary matrix for the purification of recombinant proteins, particularly those with polyhistidine (His-tag) or other metal-binding tags.
• Enzyme Purification: Isolation of specific enzymes from complex biological mixtures for research and diagnostic kit manufacturing.
• Antibody Fragment Purification: Downstream processing of antibody fragments (e.g., Fabs, scFvs) in therapeutic development.
• Biopharmaceutical Production: Critical step in the purification train for monoclonal antibodies and other biologics under GMP conditions.
• Research & Development: Used in academic and industrial R&D for protein characterization, interaction studies, and sample preparation for analysis.
• Diagnostic Reagent Manufacturing: Purification of high-value proteins used as key components in immunoassays and diagnostic tests.

Basic Information

Product Name	Tris(Carboxymethyl)Ethylenediamine-Sepharose
CAS No.	84680-76-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Tris(carboxymethyl)ethylenediamine-Sepharose; TCE-Sepharose; Sepharose-TCE; Chelating Sepharose; Iminodiacetic Acid (IDA) Sepharose Analog; Metal Chelate Affinity Medium; His-Tag Purification Resin; Ni-NTA Sepharose Alternative; TCED-Sepharose
EINECS	Contact for details

Quality Control

Our Tris(Carboxymethyl)Ethylenediamine-Sepharose is manufactured under strict quality management systems. Each batch undergoes rigorous testing for key performance parameters including ligand density, binding capacity, flow characteristics, and absence of microbial contamination to ensure consistency and reliability for critical separations. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in 20% ethanol or an appropriate storage solution as specified. Do not freeze. Keep the suspension homogeneous and avoid drying out the matrix.

Specification

Item	Specification
Appearance	White suspension
Matrix	Cross-linked 4% or 6% Agarose (Sepharose)
Ligand	Tris(Carboxymethyl)Ethylenediamine
Particle Size	45-165 μm
Binding Capacity	>20 mg His-tagged protein / mL resin
Maximum Flow Rate	Contact for details
pH Stability	3-12 (long term), 2-13 (short term)
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.