Description

Diethyl[2-Hydroxypropyl]Aminoethyl Sephadex is a functionalized, cross-linked dextran gel derivative designed for advanced chromatographic separations. This specialized material is engineered to provide high-resolution purification and fractionation of biomolecules, including proteins, enzymes, and nucleic acids, based on ion-exchange mechanisms. It is an essential tool for research laboratories, pharmaceutical development, and biotechnology companies requiring precise and scalable downstream processing solutions.

Application

• Ion-Exchange Chromatography (IEX): Primary application for the purification of charged biomolecules such as proteins, peptides, and enzymes in both analytical and preparative scales.
• Biopharmaceutical Downstream Processing: Critical step in the purification of therapeutic proteins, monoclonal antibodies, and vaccines to achieve high purity and yield.
• Enzyme Isolation and Purification: Used to separate and concentrate specific enzymes from complex biological mixtures for research and industrial biocatalysis.
• Nucleic Acid Fractionation: Effective for the separation of different forms of DNA and RNA, including plasmid purification and removal of contaminants.
• Laboratory-Scale Research: A versatile matrix for method development, process optimization, and small-scale purification in academic and R&D settings.
• Process Development and Scale-Up: Serves as a reliable medium for scaling chromatographic processes from laboratory to pilot and production scale.

Basic Information

Product Name	Diethyl[2-Hydroxypropyl]Aminoethyl Sephadex
CAS No.	83382-89-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	DEAE-Sephadex (Diethylaminoethyl Sephadex); Diethyl(2-hydroxypropyl)aminoethyl-Sephadex; 2-Hydroxypropyl-diethylaminoethyl Sephadex; Aminoethyl Sephadex derivative; DEAE-functionalized dextran gel; Anion exchanger Sephadex; Cross-linked dextran ion exchanger; Chromatography medium DEAE-Sephadex
EINECS	Contact for details

Quality Control

Our Diethyl[2-Hydroxypropyl]Aminoethyl Sephadex is manufactured under strict quality management systems. Each batch undergoes rigorous testing to ensure consistent performance, including assessments of ion-exchange capacity, particle size distribution, and swelling characteristics. Certificates of Analysis (COA) detailing lot-specific specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed when not in use to prevent moisture uptake, which can affect performance. Avoid exposure to extreme temperatures and humidity.

Specification

Item	Specification
Appearance	White to off-white free-flowing powder/beads
Ion-Exchange Capacity	Contact for details
Particle Size Range	Contact for details
Swelling Volume	Contact for details
pH Stability Range	Contact for details
Maximum Operating Pressure	Contact for details
Microbiological Bioburden	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.