Description

Sephacryl(R) S-300-HR, For Chromatography is a high-resolution, beaded, cross-linked allyl dextran gel filtration medium designed for the separation of biomolecules. This product is essential for achieving precise fractionation of proteins, polysaccharides, and other macromolecules based on their size in aqueous solutions. It is a critical tool for researchers and process development scientists in the biotechnology, pharmaceutical, and life sciences industries who require reliable, high-performance chromatography for purification, analysis, and quality control.

Application

• Size-Exclusion Chromatography (SEC) of proteins, peptides, and enzymes in aqueous buffers.
• Purification and desalting of monoclonal antibodies, recombinant proteins, and other biopharmaceuticals.
• Fractionation of polysaccharides, dextrans, and other high molecular weight polymers.
• Analysis of molecular weight distribution and aggregation states of biomolecules.
• Process-scale purification in downstream bioprocessing for manufacturing.
• Gel filtration in FPLC and HPLC systems for analytical and preparative separations.
• Buffer exchange and removal of small molecules from protein samples.

Basic Information

Product Name	Sephacryl S-300-HR, For Chromatography
CAS No.	82785-74-8
Molecular Formula	Cross-linked polymer (Allyl dextran and N,N'-methylenebisacrylamide)
Molecular Weight	Not applicable (polymeric resin)
Synonyms	Sephacryl S-300 High Resolution; Sephacryl S-300 HR; Allyl Dextran Cross-linked Gel; Size Exclusion Chromatography Gel S-300; Gel Filtration Medium S-300-HR; Dextran Allyl Ether Crosspolymer; Chromatography Resin S-300; SEC Medium Sephacryl S300HR
EINECS	Contact for details

Quality Control

Our Sephacryl S-300-HR is manufactured under a quality management system and undergoes rigorous testing to ensure batch-to-batch consistency and performance for chromatography. Key parameters such as particle size distribution, exclusion limits, flow rate, and chemical stability are verified. A Certificate of Analysis (COA) detailing specific lot specifications is available upon request to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). The product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent moisture uptake, which can affect chromatographic performance. Avoid freezing.

Specification

Item	Specification
Appearance	White to off-white beads
Particle Size	25-75 μm (mean)
Exclusion Limit (Globular Proteins)	~1.5 x 10^6 Da
Separation Range (Globular Proteins)	10,000 - 1,500,000 Da
Matrix	Cross-linked allyl dextran
Chemical Stability	Stable in aqueous buffers, 0.1M NaOH, and common chromatographic solvents
Maximum Operating Pressure	0.3 MPa (3 bar, 43 psi)
pH Stability (long term)	2 - 11
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.