Description

Affi-Gel 15 is a high-performance, activated affinity chromatography support designed for the covalent immobilization of ligands containing primary amino groups. This product is essential for researchers and process developers requiring robust, high-capacity matrices for protein purification, antibody isolation, and enzyme immobilization. It is a critical tool for biotechnology, pharmaceutical development, and life science research applications globally.

Application

• Antibody Purification: Immobilization of Protein A, Protein G, or specific antigens for the isolation of monoclonal and polyclonal antibodies from serum, ascites, or cell culture supernatants.
• Enzyme Immobilization: Covalent coupling of enzymes for biocatalysis, creating reusable catalysts for industrial synthesis and analytical applications.
• Receptor-Ligand Studies: Preparation of affinity columns for studying protein-protein interactions, receptor binding assays, and signal transduction pathways.
• Glycoprotein Isolation: Immobilization of lectins (e.g., Concanavalin A) for the selective purification of glycoproteins and polysaccharides.
• Nucleic Acid Binding Protein Purification: Coupling of specific DNA or RNA sequences for the isolation of transcription factors and other nucleic acid-binding proteins.
• Process-Scale Chromatography: Used in the development and scaling of downstream purification processes for therapeutic proteins and vaccines.

Basic Information

Product Name	Affi-Gel 15
CAS No.	79920-18-6
Molecular Formula	N/A (Functionalized cross-linked agarose bead)
Molecular Weight	N/A (Macromolecular support)
Synonyms	N-Hydroxysuccinimide ester-activated agarose; NHS-activated agarose; Affi-Gel® 15; Activated CH Sepharose 4B; N-Succinimidyl ester agarose; Carbonyldiimidazole-activated agarose (related chemistry); Activated beaded agarose for affinity chromatography.
EINECS	Contact for details

Quality Control

Our Affi-Gel 15 is manufactured under strict quality systems to ensure batch-to-batch consistency for critical research and process applications. Each lot is tested for key performance parameters including active group density, bead size distribution, flow characteristics, and coupling capacity using standard ligands. A Certificate of Analysis (COA) detailing these specifications is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store the supplied suspension at 2-8°C. Do not freeze. Under these recommended conditions, the product is stable for the duration indicated on the label. Keep the container sealed to prevent evaporation and microbial contamination.

Specification

Item	Specification
Matrix	Cross-linked beaded agarose
Active Group	N-Hydroxysuccinimide ester
Bead Size	45-165 μm (wet)
Coupling Capacity	>15 mg human serum albumin per ml gel
Exclusion Limit	>2 x 10⁷ Daltons for globular proteins
pH Stability	2-11 (long term); 2-9 (coupling)
Storage Solution	Isopropanol/water
Identification (IR)	Conforms to structure

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.