Description

Phenyl-Sepharose is a hydrophobic interaction chromatography (HIC) medium, widely recognized for its high selectivity and capacity in the purification of biomolecules. This affinity matrix is specifically designed for the separation and purification of proteins, peptides, and nucleic acids based on their surface hydrophobicity. It is an essential tool for researchers and process development scientists in the biopharmaceutical, diagnostic, and life science research sectors, enabling critical downstream processing steps.

Application

• Purification of antibodies and antibody fragments (e.g., mAbs, Fabs, scFvs) from complex biological mixtures.
• Separation of recombinant proteins expressed in E. coli or mammalian cell culture systems.
• Fractionation of plasma proteins, including serum albumin and immunoglobulins.
• Capture and polishing step in multi-column chromatography processes for high-purity biotherapeutic production.
• Removal of aggregates and misfolded proteins from final drug substance formulations.
• Research-scale purification of enzymes, hormones, and other hydrophobic biomolecules in academic and industrial labs.
• Process development and optimization for scaling up cGMP manufacturing of biologics.

Basic Information

Product Name	Phenyl-Sepharose
CAS No.	71822-39-4
Molecular Formula	Not applicable (Chromatography resin)
Molecular Weight	Not applicable
Synonyms	Phenyl Sepharose 6 Fast Flow (low sub); Phenyl Sepharose High Performance; Phenyl-Sepharose CL-4B; Phenyl-Sepharose 6 FF (high sub); Phenyl agarose; Phenyl hydrophobic chromatography medium; Phenyl sepharose resin; Phenyl sepharose fast flow
EINECS	Contact for details

Quality Control

Our Phenyl-Sepharose chromatography media are produced under a strict quality management system. Each lot is tested for critical performance parameters including ligand density, particle size distribution, flow properties, and binding capacity for standard proteins. Certificates of Analysis (COA) detailing these specifications are provided with every shipment. We support applications requiring GMP-compliant documentation and can supply resin suitable for use in regulated biopharmaceutical manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store the supplied suspension at 2-8°C. Do not freeze. For long-term storage of packed columns, use an appropriate storage solution (e.g., 20% ethanol) as recommended to prevent microbial growth and maintain performance. Keep away from strong oxidizing agents.

Specification

Item	Specification
Matrix	Cross-linked 6% agarose
Ligand	Phenyl group
Particle Size	45-165 μm (mean 90 μm)
Ligand Density	Low Sub: ~20 μmol/mL; High Sub: ~40 μmol/mL
Maximum Flow Rate	≥ 700 cm/h (at 20°C, 10 cm bed height)
Chemical Stability	Stable in all common aqueous buffers, 1M NaOH, 8M urea, 6M guanidine HCl
pH Stability	2-13 (long term); 1-14 (short term)
Storage Solution	20% Ethanol in 1M NaCl

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.