Description

Adenosine Monophosphate-Sepharose is a high-performance affinity chromatography medium, where adenosine 5'-monophosphate (5'-AMP) is covalently immobilized onto a cross-linked agarose (Sepharose) matrix. This product is specifically engineered for the selective purification and isolation of enzymes and proteins that bind nucleotide cofactors, such as dehydrogenases, kinases, and synthetases. It is an essential tool for researchers and process development scientists in biopharmaceutical production, academic biochemistry research, and diagnostic reagent manufacturing who require high-purity, functionally active proteins.

Application

• Affinity Purification of NAD(P)+-Dependent Enzymes: For the single-step isolation of dehydrogenases, reductases, and other enzymes from complex biological mixtures like cell lysates or fermentation broths.
• Biopharmaceutical Downstream Processing: Critical in the purification process of therapeutic enzymes and recombinant proteins in GMP manufacturing environments.
• Academic & Industrial Research: Used in laboratories for studying enzyme kinetics, protein-protein interactions, and cofactor binding mechanisms.
• Diagnostic Kit Component Manufacturing: Serves as a purification step for enzymes used in clinical diagnostic assays and test kits.
• Proteomics and Protein Characterization: Aids in the identification and study of novel nucleotide-binding proteins from various biological sources.
• Enzyme Immobilization Support: The matrix can be used to immobilize target enzymes for use in biocatalysis or continuous flow reactor systems.

Basic Information

Item	Details
Product Name	Adenosine Monophosphate-Sepharose
CAS No.	71330-40-0
Molecular Formula	N/A (Composite Material)
Molecular Weight	N/A (Composite Material)
Synonyms	5'-AMP-Sepharose; Adenosine-5'-monophosphate-Sepharose 4B; AMP-Sepharose 4B; 5'-Adenylic Acid-Sepharose; Sepharose-AMP; Adenosine Monophosphate Agarose; Affinity Chromatography Medium AMP; NAD(P)+-Affinity Gel
EINECS	Contact for details

Quality Control

Our Adenosine Monophosphate-Sepharose is produced under a stringent quality management system. Each lot is tested to ensure consistent ligand density, binding capacity, and matrix stability. Certificates of Analysis (COA) are provided, detailing parameters such as ligand concentration, gel volume, and binding capacity for a standard enzyme (e.g., lactate dehydrogenase). We adhere to relevant quality guidelines for bioprocessing materials to support your regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store as a 50% slurry in a 20% ethanol solution at 2-8°C. Do not freeze. Under these conditions, the product is stable for several years. The suspension contains a bacteriostatic agent to prevent microbial growth.

Specification

Item	Specification
Matrix	Cross-linked 4% Agarose (Sepharose 4B)
Ligand	Adenosine 5'-Monophosphate (5'-AMP)
Ligand Density	1 - 3 µmol AMP / mL settled gel
Binding Capacity	> 5 mg L-Lactate Dehydrogenase / mL settled gel
Particle Size	45 - 165 µm
Storage Solution	20% Ethanol, 0.1 M NaCl
pH Stability	3 - 12 (long term); 2 - 14 (short term)
Maximum Flow Pressure	0.3 MPa, 3 bar

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.