Description

Thiol-Sepharose is a high-performance affinity chromatography medium designed for the purification of thiol-containing proteins and peptides via covalent chromatography. This product is essential for researchers and process developers requiring high-capacity, selective capture and immobilization of biomolecules. It is a critical tool in downstream bioprocessing for the pharmaceutical, biotechnology, and life sciences industries, enabling the purification of enzymes, antibodies, and other therapeutic proteins.

Application

• Affinity Chromatography: Primary application for the purification of thiol-containing proteins, peptides, and enzymes through reversible covalent binding.
• Protein Immobilization: Covalent coupling of enzymes or antibodies to create stable, reusable biocatalysts or affinity ligands for further purification steps.
• Disulfide Bond Reduction Studies: Used to capture and study proteins with reduced cysteine residues or to isolate specific protein variants.
• Antibody Fragment Purification: Effective for purifying Fab' and other antibody fragments that contain free thiol groups.
• Recombinant Protein Production: A key step in downstream processing for recombinant proteins engineered with cysteine tags or native thiol groups.
• Sample Preparation for Proteomics: Selective isolation of cysteine-containing peptides for mass spectrometry analysis.

Basic Information

Product Name	Thiol-Sepharose
CAS No.	69552-82-5
Molecular Formula	N/A (Chromatography Resin)
Molecular Weight	N/A (Chromatography Resin)
Synonyms	Thiopropyl Sepharose 6B; Sepharose 6B activated with 2-thiopyridyl groups; Activated Thiol Sepharose 4B; Thiol-activated Agarose; Covalent Chromatography Medium; Thiol-reactive Sepharose; 2-Pyridyldithiol Sepharose; Glutathione-affinity resin analog (for coupling)
EINECS	Contact for details

Quality Control

Our Thiol-Sepharose is manufactured under a strict quality management system. Each lot is tested for key performance parameters including ligand density, binding capacity, flow properties, and absence of microbial contamination to ensure batch-to-batch consistency. Certificates of Analysis (COA) detailing these specifications are provided with every shipment. Production adheres to cGMP guidelines where applicable, supporting use in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store as a 20% ethanol suspension at 2-8°C (36-46°F) to prevent microbial growth and maintain stability. Do not freeze. The product is light-sensitive (store away from light).

Specification

Item	Specification
Matrix	Cross-linked 6% Agarose (Sepharose 6B)
Ligand	2-Thiopyridyl
Ligand Density	Contact for details
Binding Capacity	Contact for details
Particle Size	45-165 μm (wet)
Storage Solution	20% Ethanol
pH Stability	2-11 (long term); 3-9 (working)
Maximum Pressure	0.1 MPa
Microbial Bioburden	Absent

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.