Description

Bleomycin Bapp is a glycopeptide antitumor antibiotic derived from *Streptomyces verticillus*, structurally related to bleomycin A2 and B2 but distinguished by its unique terminal amine moiety. It serves as a critical research-grade compound for oncology drug development, mechanism-of-action studies, and structure-activity relationship (SAR) profiling in academic and pharmaceutical R&D laboratories. This high-purity material is essential for preclinical cytotoxicity assays, DNA cleavage analysis, and targeted delivery system validation. Bleomycin Bapp CAS NO 69177-41-9 is supplied under stringent quality controls to ensure batch-to-batch reproducibility and experimental fidelity.

Application

• Oncology research: In vitro and in vivo evaluation of antiproliferative activity against human carcinoma cell lines (e.g., A549, HeLa, MCF-7)
• Mechanistic studies: Investigation of sequence-selective DNA strand scission and oxidative damage pathways
• Drug conjugate development: As a payload in antibody–drug conjugates (ADCs) and peptide–drug conjugates targeting tumor-associated antigens
• Biomarker discovery: Use in controlled genotoxicity models to identify DNA repair pathway modulation signatures
• Reference standard: Quantitative calibration in HPLC and LC-MS/MS methods for bleomycin family analytes
• Pharmaceutical stability testing: Forced degradation studies under oxidative, photolytic, and hydrolytic stress conditions
• Formulation science: Excipient compatibility screening for lyophilized antitumor injectables
• Regulatory dossier support: Impurity profiling and reference material qualification per ICH Q5 and Q7 guidelines

Basic Information

Product Name	Bleomycin Bapp
CAS No.	69177-41-9
Molecular Formula	C55H84N17O21S2
Molecular Weight	1415.48 g/mol
Synonyms	Bleomycin Bapp; Bleomycin Bapp (Bleomycin B1 analog); Bleomycin B1-aminopropyl; N-(3-Aminopropyl)bleomycin B1; Bleomycin B1-NH(CH2)3NH2; BLM-Bapp; Bleomycin B-app; Bleomycin B-aminopropyl; Bleomycin B-AP; Bleomycin B-APA
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch is verified for identity (IR, HPLC retention time), assay (HPLC), related substances (HPLC), residual solvents (GC), heavy metals (USP <231>), and microbiological purity (USP <61>). Testing aligns with ACS Reagent Grade and EP 11.0 specifications for biologically active peptides and antibiotics.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (nitrogen or argon) to prevent oxidation. Due to hygroscopic nature, maintain low humidity (<30% RH) and avoid repeated exposure to ambient air. Handle under dry nitrogen when opening vials.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 95.0%
Related Substances (HPLC)	Single impurity ≤ 1.0%; Total impurities ≤ 3.0%
Residual Solvents (GC)	MeOH ≤ 3000 ppm; Acetonitrile ≤ 410 ppm; Water ≤ 5.0%
Heavy Metals	≤ 20 ppm
Loss on Drying	≤ 8.0%
Microbial Enumeration	Total aerobic count ≤ 100 CFU/g; Absence of E. coli, Salmonella, and Staphylococcus aureus

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.