Description

Phleomycin is a glycopeptide antibiotic complex derived from *Streptomyces verticillus*, known for its potent ability to cleave DNA. This compound is a critical research tool for studying DNA damage and repair mechanisms, as well as for selecting genetically engineered cells. It is primarily utilized in molecular biology and biochemical research laboratories for applications such as cell line selection, mutagenesis studies, and investigating the cellular response to DNA strand breaks.

Application

• Molecular Biology Research: Used as a selective agent in cell culture for the isolation of recombinant cells, particularly in yeast and bacterial genetics.
• DNA Damage Studies: Employed to induce double-strand breaks in DNA to study repair pathways, genomic instability, and apoptosis.
• Antibiotic Mechanism Research: Serves as a model compound for investigating the action of bleomycin-family antibiotics and developing novel therapeutics.
• Cell Line Development: Applied in biopharmaceutical research for the selection and maintenance of transfected mammalian cell lines expressing resistance genes.
• Microbiological Assays: Used in agar diffusion tests to assess microbial susceptibility or to screen for antibiotic-producing organisms.

Basic Information

Product Name	Phleomycin
CAS No.	11006-33-0
Molecular Formula	C55H84N17O21S2 (for Phleomycin D1, a major component)
Molecular Weight	Approx. 1400 g/mol (for the complex; varies by component)
Synonyms	Phleomycin complex; Phleomycin from Streptomyces verticillus; Bleomycin phleomycin; NSC-113373; Antibiotic BLR 02; Antibiotic LA 7017; Phleomycin D1; Phleomycin sulfate
EINECS	234-245-9

Quality Control

Our Phleomycin is produced and handled under strict quality management protocols. Each batch undergoes comprehensive analytical testing to ensure identity, purity, and biological activity. A detailed Certificate of Analysis (COA) is provided with each shipment, reporting key parameters such as potency (units/mg), purity by HPLC, and endotoxin levels where applicable. We adhere to cGMP guidelines for the manufacture of research-grade biochemicals to guarantee consistent performance and reliable results in your critical experiments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive). For long-term stability, it is recommended to store desiccated. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Light yellow to off-white powder
Identification (HPLC)	Conforms to reference standard
Potency (Microbiological Assay)	≥ 90%
Purity (HPLC)	≥ 85% (Total phleomycin components)
Water Content (KF)	≤ 5.0%
Endotoxin (LAL)	< 10 EU/mg (for cell culture grade)
Solubility	Soluble in water, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.