Description

Deae-Sepharose Cl-6B is a beaded, cross-linked agarose matrix functionalized with diethylaminoethyl (DEAE) groups, making it a robust weak anion exchanger for preparative chromatography. This product is essential for the scalable purification of a wide range of biomolecules, including proteins, nucleic acids, and enzymes, under mild conditions. It is a critical tool for researchers and process development scientists in the biotechnology, pharmaceutical, and life science industries who require high capacity and excellent flow properties for downstream processing.

Application

• Protein Purification: Primary application for the isolation and purification of enzymes, antibodies, and other proteins from complex biological mixtures.
• Nucleic Acid Separation: Effective for the fractionation and purification of plasmid DNA, RNA, and oligonucleotides.
• Enzyme Immobilization: Serves as a solid support for the covalent immobilization of enzymes for biocatalysis and industrial processes.
• Vaccine Development: Used in downstream processing steps for the purification of viral vectors and recombinant vaccine antigens.
• Diagnostic Reagent Production: Employed in the purification of key components for immunoassays and diagnostic kits.
• Process Development & Scale-up: Ideal for method development in laboratory settings with direct scalability to industrial manufacturing columns.

Basic Information

Product Name	Deae-Sepharose Cl-6B
CAS No.	68652-08-4
Molecular Formula	Not applicable (Chromatographic resin)
Molecular Weight	Not applicable
Synonyms	DEAE-Sepharose CL-6B; Diethylaminoethyl-Sepharose CL-6B; DEAE Agarose CL-6B; Sepharose CL-6B DEAE; Weak Anion Exchanger CL-6B; Chromatography Media DEAE-Sepharose; Cross-linked DEAE Agarose; 6% Cross-linked DEAE Agarose Beads
EINECS	Contact for details

Quality Control

Our Deae-Sepharose Cl-6B is manufactured under a strict quality management system. Each batch is tested for critical parameters including ionic capacity, particle size distribution, flow rate, and binding capacity for standard proteins to ensure consistent, high-performance separation. Certificates of Analysis (COA) detailing these specifications are available upon request to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in the supplied 20% ethanol storage solution. Do not freeze. For long-term storage, ensure the container is sealed to prevent evaporation and microbial contamination. Keep away from strong acids and organic solvents.

Specification

Item	Specification
Matrix	Cross-linked 6% agarose beads
Functional Group	Diethylaminoethyl (DEAE)
Ionic Capacity	100-160 μmol Cl⁻/ml settled gel
Particle Size	45-165 μm
Exclusion Limit	~4x10⁶ for globular proteins
pH Stability	2-14 (long term), 2-12 (working)
Storage Solution	20% Ethanol
Binding Capacity (BSA)	≥ 100 mg/ml settled gel

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.