Description

Sephadex-Qae A-25, Ion-Exchange Resin, 40-120Micron is a strong anion exchange chromatography medium based on a cross-linked dextran matrix. This product is essential for the high-resolution separation and purification of biomolecules, particularly nucleic acids, proteins, and other charged species in research and process-scale applications. It is a critical tool for laboratories and production facilities in the biotechnology, pharmaceutical, and life sciences sectors requiring reliable, reproducible purification performance.

Application

• Nucleic Acid Purification: Separation and purification of plasmid DNA, RNA, oligonucleotides, and other nucleic acids from complex biological mixtures.
• Protein & Peptide Separation: Fractionation of proteins, enzymes, antibodies, and peptides based on charge differences in downstream bioprocessing.
• Desalting & Buffer Exchange: Efficient removal of salts, dyes, and other small ionic contaminants from biomolecule solutions.
• Enzyme Immobilization: Serving as a solid support matrix for the covalent attachment of enzymes for biocatalysis and industrial applications.
• Analytical Chromatography: Use in laboratory-scale column chromatography for analytical method development and quality control testing.
• Process-Scale Chromatography: Scalable purification in preparative and production-scale columns for manufacturing therapeutic biologics and diagnostics.

Basic Information

Product Name	Sephadex-Qae A-25, Ion-Exchange Resin, 40-120Micron
CAS No.	65637-73-2
Molecular Formula	Contact for details
Molecular Weight	Not applicable (Polymeric resin)
Synonyms	QAE Sephadex A-25; Quaternary Aminoethyl Sephadex A-25; DEAE Sephadex derivative; Strong Anion Exchange Dextran Gel; QAE-A25; Anion Exchange Chromatography Media; Dextran-based QAE resin; QAE-Sephadex A-25 (40-120 μm)
EINECS	Contact for details

Quality Control

Our Sephadex-Qae A-25 resin is manufactured under a rigorous quality management system. Each lot is tested for critical performance parameters including ionic capacity, particle size distribution, flow characteristics, and purity to ensure consistent chromatographic performance and reproducibility. Certificates of Analysis (COA) detailing these specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent moisture uptake, which can affect performance. Keep away from strong bases and organic solvents.

Specification

Item	Specification
Appearance	White to off-white free-flowing beads
Particle Size	40 - 120 micron
Matrix	Cross-linked dextran
Functional Group	Quaternary aminoethyl (QAE)
Ionic Form (shipped as)	Chloride (Cl⁻)
Ionic Capacity	Contact for details
Exclusion Limit	Contact for details
pH Stability (operational)	2 - 12
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.