Description

Sephacryl S-200 is a high-resolution, beaded gel filtration medium designed for the separation of biomolecules. It is a cross-linked allyl dextran matrix that provides excellent chemical and physical stability, making it ideal for demanding laboratory and process-scale applications. This product is essential for researchers and manufacturers in the biotechnology and pharmaceutical industries who require reliable, high-performance size-exclusion chromatography for purifying proteins, peptides, nucleic acids, and polysaccharides.

Application

• Size-exclusion chromatography (SEC) for the purification of proteins and antibodies.
• Desalting and buffer exchange of biomolecules in downstream bioprocessing.
• Separation of nucleic acids, plasmids, and oligonucleotides in molecular biology workflows.
• Fractionation of polysaccharides, dextrans, and other polymers.
• Removal of aggregates and fragments from therapeutic protein preparations.
• Process-scale purification in Good Manufacturing Practice (GMP) environments for biopharmaceuticals.
• Analytical separation for molecular weight determination of biomolecules.
• Laboratory-scale purification in academic and industrial research settings.

Basic Information

Product Name	Sephacryl S-200
CAS No.	65546-95-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Sephacryl S-200 HR; Sephacryl S-200 High Resolution; Allyl dextran cross-linked with N,N'-methylenebisacrylamide; Gel filtration medium S-200; Size exclusion chromatography medium S-200; Dextran allyl ether cross-linked polymer; Chromatography resin S-200; Bio-Gel S-200 (alternative trade name)
EINECS	Contact for details

Quality Control

Our Sephacryl S-200 is manufactured under strict quality management systems to ensure batch-to-batch consistency and performance. Each lot is tested for critical parameters including particle size distribution, exclusion limits, flow rate, and chemical stability. Certificates of Analysis (COA) documenting these specifications are available upon request to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a consistent room temperature (15-25°C). The product is stable in a wide range of aqueous buffers but should be protected from strong bases and organic solvents as indicated in its chemical properties. For long-term storage, keep the medium in the supplied preservative solution (typically 20% ethanol) to prevent microbial growth.

Specification

Item	Specification
Appearance	White, opaque beads
Matrix	Cross-linked allyl dextran
Particle Size	25-75 μm (dry)
Exclusion Limit (Globular Proteins)	~80,000 Da
Operating pH Range	2-13 (long term), 3-11 (short term)
Chemical Stability	Stable in commonly used aqueous buffers, detergents, and 0.1-0.2 M NaOH. Not stable in strong bases or organic solvents.
Maximum Pressure	0.3 MPa (3 bar, 44 psi)
Maximum Flow Rate	~30 cm/h

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.