Description

Sepharose Cl-2B is a cross-linked, beaded agarose gel designed for high-performance gel filtration chromatography. This product is essential for the gentle and effective separation of large biomolecules, such as viruses, very large proteins, and nucleic acids, based on their size. It is a critical tool for researchers and process developers in the biotechnology and pharmaceutical industries, particularly for applications requiring high flow rates and excellent chemical stability under a range of conditions.

Application

• Size-Exclusion Chromatography (SEC/GFC): Primary application for the separation and purification of high molecular weight biomolecules.
• Virus Purification: Isolation and purification of viral particles, vaccines, and viral vectors for gene therapy.
• Macromolecular Complex Analysis: Separation of protein complexes, lipoproteins, and other large assemblies.
• Nucleic Acid Fractionation: Purification of plasmid DNA, genomic DNA, and large RNA molecules.
• Process Development & Scale-up: Used from laboratory-scale research to pilot and production-scale biomanufacturing.
• Desalting & Buffer Exchange: Rapid removal of salts, dyes, or other small molecules from protein and nucleic acid samples.

Basic Information

Item	Details
Product Name	Sepharose Cl-2B
CAS No.	65099-79-8
Molecular Formula	(C12H18O9)n (Agarose polymer)
Molecular Weight	High molecular weight polymer
Synonyms	CL-2B Sepharose; Sepharose CL 2B; Cross-linked Sepharose 2B; Agarose CL-2B; 2% Cross-linked Agarose; Beaded Agarose CL-2B; Gel Filtration Medium CL-2B; Chromatography Resin CL-2B
EINECS	Contact for details

Quality Control

Our Sepharose Cl-2B is manufactured under a rigorous quality management system. Each lot is tested to ensure consistent performance characteristics, including bead size distribution, exclusion limits, flow rate, and chemical stability. Certificates of Analysis (COA) detailing these specifications are available for every batch to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). Avoid freezing. For long-term storage as a suspension, use a preservative solution as recommended (e.g., 20% ethanol).

Specification

Item	Specification
Matrix	Cross-linked 2% Agarose Beads
Bead Size (dry)	40 - 165 μm
Exclusion Limit (Globular Protein)	~ 40 x 10⁶ Da
Operating pH Range	3 - 14 (short term); 4 - 13 (long term)
Chemical Stability	Stable in most aqueous buffers, detergents, and 8M urea. Stable in 0.1M NaOH.
Maximum Linear Flow Rate	> 30 cm/h (XK 16/70 column)
Preservative	Typically supplied in 20% ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.