Description

Bleomycin A2, Hydrochloride is a glycopeptide antitumor antibiotic derived from Streptomyces verticillus, widely used in research and clinical oncology for its DNA-cleaving activity. It delivers targeted cytotoxicity against rapidly dividing cells, making it indispensable for mechanistic cancer research and therapeutic development. Researchers in pharmaceutical R&D, academic oncology labs, and biotech CMC teams rely on high-purity Bleomycin A2, Hydrochloride CAS NO 49830-49-1 for reproducible assay performance and preclinical validation.

Application

• Preclinical anticancer drug discovery and mechanism-of-action studies (DNA strand break induction)
• Cell culture-based cytotoxicity assays in human tumor cell lines (e.g., A549, HeLa, MCF-7)
• Reference standard for HPLC and LC-MS method development and validation in quality control labs
• Component in controlled-release formulation studies for targeted chemotherapy delivery systems
• Tool compound for investigating p53-dependent apoptosis pathways and oxidative stress responses
• Supporting GMP-compliant process development for biosimilar or next-generation bleomycin analogs
• Calibration standard in regulatory submissions requiring pharmacopoeial reference materials (USP/EP)
• Quality assurance testing of commercial bleomycin sulfate formulations

Basic Information

Product Name	Bleomycin A2, Hydrochloride
CAS No.	49830-49-1
Molecular Formula	C55H84N17O21S2·HCl
Molecular Weight	1415.45 g/mol
Synonyms	Bleomycin A2 hydrochloride; Bleomycin A-II hydrochloride; BLMA2·HCl; NSC-125066; BLM-A2·HCl; Bleomycin A2 HCl salt; (2S,3R,4R,5R,6R)-6-{[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-2-({(2S,3S,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl}oxy)-3,4-dihydroxy-5-[(methylamino)carbonyl]cyclohex-5-ene-1-carboxylic acid derivative with 4-amino-N-[(1S)-1-[(2-amino-4-methylpentanoyl)amino]-2-[(3-aminopropyl)amino]-2-oxoethyl]butanamide; Bleomycin A2 monohydrochloride
EINECS	256-500-7

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. All batches meet stringent specifications for identity (IR, HPLC retention time), assay (≥95.0% by HPLC), related substances (<3.0% total impurities), residual solvents (ICH Class 3 compliant), and microbiological purity (sterile filtration option available). Testing follows USP General Chapters <1058> (Analytical Instrument Qualification) and <1225> (Validation of Compendial Procedures), supporting regulatory filings in FDA, EMA, PMDA, and NMPA jurisdictions.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (nitrogen or argon) to prevent oxidation. Due to its hygroscopic nature, maintain low-humidity conditions (<30% RH) and minimize exposure to ambient air during handling. Avoid repeated freeze-thaw cycles if stored frozen.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	95.0 – 102.0%
Related Substances	≤3.0% (single impurity ≤0.5%; total ≤3.0%)
Residual Solvents (Acetonitrile, Methanol)	≤5000 ppm each (ICH Q3C Class 3)
Water (Karl Fischer)	≤5.0%
Heavy Metals	≤20 ppm
Microbial Limits	Total aerobic count ≤100 CFU/g; absence of E. coli, S. aureus, P. aeruginosa, S. typhimurium

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.