Description

N(Alpha)-Tosylarginine-4-Nitroanilide is a specialized chromogenic substrate used in enzymatic assays. This compound is critical for the precise measurement of trypsin-like protease activity in biochemical research and diagnostic applications. It is primarily utilized by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic research institutions focused on enzymology and proteomics.

Application

• Primary Substrate for Trypsin and Trypsin-like Proteases: Used in kinetic and endpoint assays to measure enzyme activity and inhibition.
• Biochemical Research Reagent: Essential for studying protease function, enzyme kinetics, and signal transduction pathways in vitro.
• Diagnostic Kit Component: Incorporated into commercial and in-house test kits for the detection and quantification of specific proteases in clinical samples.
• Drug Discovery & Screening: Serves as a key tool in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors.
• Quality Control in Biotechnology: Employed to assess the purity and specific activity of enzyme preparations, including recombinant proteases.

Basic Information

Product Name	N(Alpha)-Tosylarginine-4-Nitroanilide
CAS No.	36186-55-7
Molecular Formula	C19H23N7O5S
Molecular Weight	461.50 g/mol
Synonyms	Tosyl-Gly-Pro-Arg-p-nitroanilide acetate salt; TGPAN; Chromogenic Substrate for Trypsin; Tos-Gly-Pro-Arg-pNA; N-α-Tosylglycyl-L-prolyl-L-arginine-4-nitroanilide acetate; Bz-Ile-Glu-Gly-Arg-pNA; Trypsin Substrate I; 4-Nitroanilide derivative of Tosylarginine
EINECS	Contact for details

Quality Control

Our N(Alpha)-Tosylarginine-4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive biochemical applications. A detailed Certificate of Analysis (COA) is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be kept under desiccant to maintain stability. Allow the sealed container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Clear, colorless solution in DMSO
Functional Activity	Suitable for enzyme assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.