Description

Esperamicin P is a highly potent enediyne antitumor antibiotic isolated from Actinomadura verrucosospora, renowned for its sequence-selective DNA cleavage activity and exceptional cytotoxicity against proliferating cancer cells. It serves as a critical molecular tool in oncology research, particularly for mechanistic studies of DNA damage response, targeted drug delivery systems, and antibody–drug conjugate (ADC) development. Researchers in pharmaceutical R&D, academic cancer biology laboratories, and biotechnology companies developing next-generation anticancer therapeutics rely on high-purity Esperamicin P CAS NO 133978-90-2 for reproducible, high-fidelity experimental outcomes.

Application

• Research-grade active pharmaceutical ingredient (API) for preclinical evaluation of enediyne-based anticancer agents
• Key payload in antibody–drug conjugates (ADCs) targeting solid tumors and hematological malignancies
• Mechanistic probe for studying site-specific double-strand DNA break induction and repair pathway inhibition
• Benchmark compound in high-throughput screening assays for novel DNA-damaging agents
• Reference standard in analytical method development (HPLC, LC-MS) for enediyne quantification and stability testing
• Tool compound for investigating p53-dependent and p53-independent apoptosis pathways
• Component in structure–activity relationship (SAR) studies of modified esperamicin analogues
• Calibration material for radiolabeled or fluorescently tagged enediyne derivatives used in cellular imaging

Basic Information

Product Name	Esperamicin P
CAS No.	133978-90-2
Molecular Formula	C54H73NO22
Molecular Weight	1144.15 g/mol
Synonyms	Esperamicin P; Esperamicin A1 derivative; (1R,2S,3R,4R,5R,6R,7S,8R,9S,10R,11S,12R,13R,14R,15S,16R)-1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16-Hexadecahydroxy-17-(2-methylpropyl)-18-oxo-19,20-dihydro-19,20-ethano-21H-benzo[a]naphth[2,1-b]azepine-21-carboxylic acid; Esperamicin P (133978-90-2); NSC 639830; ESM-P; Esperamicin analogue P
EINECS	Not listed (not assigned)

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. All batches of Esperamicin P are tested for identity (HPLC retention time match, IR spectroscopy), assay (≥95.0% by HPLC), related substances (individual impurities ≤0.5%, total impurities ≤3.0%), residual solvents (meets ICH Q3C limits), and microbiological purity (sterile filtration confirmed). Testing adheres to current Good Manufacturing Practice (cGMP) guidelines applicable to research-grade bioactive compounds.

Storage

Preserve in a tightly closed container, protected from light. Store at −20 °C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, maintain low-humidity conditions (<30% RH) and minimize exposure to ambient air during handling. Avoid repeated freeze–thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time matches reference standard
Identification (IR)	Characteristic enediyne and amino sugar absorption bands
Assay (HPLC)	≥95.0%
Related Substances	Individual ≤0.5%; Total ≤3.0%
Residual Solvents	Meets ICH Q3C Class 3 limits
Water Content (KF)	≤5.0%
Heavy Metals	≤20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.