Description

L-Lysinamide, N-[2-(7-methoxy-2-oxo-2H-1-benzopyran-4-yl)acetyl]-L-alanyl-L-valyl-L-leucyl-L-glutaminyl-L-serylglycyl-L-phenylalanyl-L-arginyl-N6-(2,4-dinitrophenyl)-L-lysyl- is a complex, synthetic peptide derivative featuring a coumarin-based fluorophore and a dinitrophenyl (DNP) label. This compound is of significant value in advanced biochemical research, particularly for its utility as a highly specific, fluorescently tagged substrate or probe in enzymatic assays and receptor binding studies. It is primarily needed by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers focused on protease activity, signal transduction pathways, and high-throughput screening applications.

Application

• Protease and Enzyme Substrate: Used as a specialized, labeled peptide substrate for studying the kinetics and inhibition of specific proteases (e.g., trypsin, thrombin-like enzymes) in biochemical assays.
• Fluorescence-Based Assay Development: Serves as a key component in developing sensitive fluorogenic or fluorescence-quenching assays for drug discovery and diagnostic platforms.
• Biomolecular Interaction Studies: Employed as a tracer or competitor in studies investigating protein-protein interactions, receptor-ligand binding, and antibody affinity using fluorescence detection methods.
• Cell Biology Research: Utilized as a tool to visualize and track specific enzymatic activity within cellular systems or tissue samples.
• Peptide Chemistry & Conjugation Reference: Acts as a model compound in methodological development for peptide synthesis, labeling techniques, and purification strategies involving complex, multi-functional peptides.

Basic Information

Product Name	L-Lysinamide, N-[2-(7-methoxy-2-oxo-2H-1-benzopyran-4-yl)acetyl]-L-alanyl-L-valyl-L-leucyl-L-glutaminyl-L-serylglycyl-L-phenylalanyl-L-arginyl-N6-(2,4-dinitrophenyl)-L-lysyl-
CAS No.	932391-88-3
Molecular Formula	C71H100N18O21
Molecular Weight	1549.67 g/mol
Synonyms	DNP-Labeled Fluorescent Peptide Substrate; 7-Methoxycoumarin-4-acetyl-AVLSQGSFRK(DNP)-NH2; AVLSQGSFRK(Dnp)-MCA; Fluorogenic Peptide Substrate with DNP Quencher; MCA-AVLSQGSFRK(Dnp)-NH2; N6-(2,4-Dinitrophenyl)-L-lysyl Peptide Derivative; Coumarin-DNP Dual-Labeled Peptide
EINECS	Contact for details

Quality Control

This high-purity biochemical is manufactured under controlled conditions and undergoes rigorous analytical characterization to ensure batch-to-batch consistency and identity. Quality is verified via HPLC, MS, and NMR to confirm purity and structural integrity. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and related substance profiles is provided with each batch to support your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular weight
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 80.0% (by weight)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.